Bacterial surfaces in geochemistry : how can x-ray photoelectron spectroscopy help?

• Processes occurring at surfaces and interfaces are very important in environmental systems, necessitating surface-specific characterization tools that can help us understand processes at and specific properties of surfaces and interfaces, and their role in biogeochemical systems. This chapter describes the use and application of X-ray photoelectron spectroscopy (XPS) to study interfacial processes of relevance for geomicrobiology. Examples are given from studies determining cell wall composition, acid–base properties, cell surface charge, metal adsorption onto bacterial cells, and bacterial surface–induced precipitation of secondary minerals. As XPS is an ultrahigh-vacuum technique, several sample preparation methods have been applied to enable analysis of bacterial samples, including analysis of freeze-dried samples as well as frozen bacterial suspensions. These are described and discussed alongside advantages and disadvantages of different approaches, with a special focus on fast-freezing and the cryogenic technique.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

NATURVETENSKAP  -- Kemi -- Materialkemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Materials Chemistry (hsv//eng)

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

NATURVETENSKAP  -- Kemi -- Annan kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Other Chemistry Topics (hsv//eng)

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