Arabidopsis thaliana G3BP Ortholog Rescues Mammalian Stress Granule Phenotype across Kingdoms

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Fältnamn	Indikatorer	Metadata
000		02534naa a2200337 4500
001		oai:prod.swepub.kib.ki.se:146995746
003		SwePub
008		240811s2021 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1469957462 URI
024	7	a https://doi.org/10.3390/ijms221262872 DOI
040		a (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Reuper, H4 aut
245	1 0	a Arabidopsis thaliana G3BP Ortholog Rescues Mammalian Stress Granule Phenotype across Kingdoms
264		c 2021-06-11
264	1	b MDPI AG,c 2021
520		a Stress granules (SGs) are dynamic RNA–protein complexes localized in the cytoplasm that rapidly form under stress conditions and disperse when normal conditions are restored. The formation of SGs depends on the Ras-GAP SH3 domain-binding protein (G3BP). Formations, interactions and functions of plant and human SGs are strikingly similar, suggesting a conserved mechanism. However, functional analyses of plant G3BPs are missing. Thus, members of the Arabidopsis thaliana G3BP (AtG3BP) protein family were investigated in a complementation assay in a human G3BP knock-out cell line. It was shown that two out of seven AtG3BPs were able to complement the function of their human homolog. GFP-AtG3BP fusion proteins co-localized with human SG marker proteins Caprin-1 and eIF4G1 and restored SG formation in G3BP double KO cells. Interaction between AtG3BP-1 and -7 and known human G3BP interaction partners such as Caprin-1 and USP10 was also demonstrated by co-immunoprecipitation. In addition, an RG/RGG domain exchange from Arabidopsis G3BP into the human G3BP background showed the ability for complementation. In summary, our results support a conserved mechanism of SG function over the kingdoms, which will help to further elucidate the biological function of the Arabidopsis G3BP protein family.
700	1	a Gotte, B4 aut
700	1	a Williams, L4 aut
700	1	a Tan, TJC4 aut
700	1	a McInerney, GMu Karolinska Institutet4 aut
700	1	a Panas, MDu Karolinska Institutet4 aut
700	1	a Krenz, B4 aut
710	2	a Karolinska Institutet4 org
773	0	t International journal of molecular sciencesd : MDPI AGg 22:12q 22:12x 1422-0067
856	4	u https://doi.org/10.3390/ijms22126287
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:146995746
856	4 8	u https://doi.org/10.3390/ijms22126287

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Av författaren/redakt...: Reuper, H; Gotte, B; Williams, L; Tan, TJC; McInerney, GM; Panas, MD; visa fler...; Krenz, B; visa färre...

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Av lärosätet: Karolinska Institutet

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