WFRF:(Ruetschi Ulla)
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Regional assignment of the human 4-hydroxyphenylpyruvate dioxygenase gene (HPD) to 12q24-->qter by fluorescence in situ hybridization.
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- Stenman, Göran, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin , Avdelningen för patologi,Institute of Laboratory Medicine, Dept of Pathology
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- Röijer, E (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin , Avdelningen för patologi,Institute of Laboratory Medicine, Dept of Pathology
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- Rüetschi, Ulla, 1962 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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- (creator_code:org_t)
- 1995
- 1995
- English.
- In: Cytogenetics and cell genetics. - 0301-0171. ; 71:4, s. 374-6
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Using a panel of human-rodent somatic cell hybrids, we have previously mapped the gene (HPD, previously called PPD) encoding 4-hydroxyphenylpyruvate dioxygenase to the distal half of the long arm of human chromosome 12, region q14-->qter. To obtain a genomic probe useful for fluorescence in situ hybridization (FISH) analysis we screened a human leukocyte genomic library and isolated a 13.4-kb phage clone, which by restriction fragment and sequence analyses was shown to contain exons 1-10 of HPD and approximately 2-kb upstream sequences. We now report the subregional localization of HPD to 12q24-->qter based on two color FISH analysis employing this clone.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- 4-Hydroxyphenylpyruvate Dioxygenase
- genetics
- Animals
- Base Sequence
- Blotting
- Southern
- Chromosome Mapping
- Chromosomes
- Human
- Pair 12
- genetics
- ultrastructure
- DNA
- Complementary
- Exons
- genetics
- Humans
- In Situ Hybridization
- Fluorescence
- Mice
- Molecular Sequence Data
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Dellsén, A
- Rymo, Lars, 1940
- Lindstedt, Sven
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- University of Gothenburg
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