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Nitric oxide-depend...
Nitric oxide-dependent protein synthesis in parotid and submandibular glands of anaesthetized rats upon sympathetic stimulation or isoprenaline administration
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- Sayardoust, Shariel (författare)
- Department of Pharmacology, Sahlgrenska Academy Göteborg University, Göteborg, Sweden
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- Ekström, J. (författare)
- Department of Pharmacology, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
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(creator_code:org_t)
- 2004-02-19
- 2004
- Engelska.
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Ingår i: Experimental Physiology. - : John Wiley & Sons. - 0958-0670 .- 1469-445X. ; 89:2, s. 219-227
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In anaesthetized female rats, the β-adrenoceptor agonist isoprenaline was intravenously infused (20 μg kg-1 min-1) for 30 min or the ascending cervical sympathetic nerve trunk was intermittently stimulated (50 Hz, 1 s every tenth second) on one side for 30 min. The incorporation of [3H]leucine into trichloroacetic acid (TCA)-insoluble material was used as an index of protein synthesis. In response to isoprenaline, the [3H]leucine incorporation increased by 79% in the parotid glands and by 82% in the submandibular glands. The neuronal type NO-synthase inhibitor N-PLA, reduced (P < 0.001) this response to 26% and 20%, respectively. Sympathetic stimulation under α-adrenoceptor blockade increased the [3H]leucine incorporation by 192% in the parotid glands and by 35% in the submandibular glands. N-PLA reduced the corresponding percentage figures to 86% (P < 0.01) and 8% (P < 0.05). When tested in the parotid glands, the non-selective NO-synthase inhibitor L-NAME reduced (P < 0.01) the nerve-evoked response to 91%. The increase in [3H]leucine incorporation in response to sympathetic stimulation under β-adrenoceptor blockade was not affected by N-PLA in the parotid (139% versus 144%) and submandibular glands (39% versus 34%). In non-stimulated glands, the [3H]leucine incorporation was not influenced by the NO-synthase inhibitors. In conclusion, β-adrenoceptor mediated salivary gland protein synthesis is largely dependent on NO generation by neuronal type NO-synthase, most likely of parenchymal origin.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- beta adrenergic receptor blocking agent
- isoprenaline
- leucine
- n(g) nitroarginine methyl ester
- nitric oxide
- nitric oxide synthase
- nitric oxide synthase inhibitor
- trichloroacetic acid
- adrenergic stimulation
- animal tissue
- article
- beta adrenergic receptor blocking
- controlled study
- enzyme inhibition
- evoked response
- female
- nerve stimulation
- nonhuman
- parotid gland
- protein synthesis
- rat
- statistical analysis
- statistical significance
- submandibular gland
- sympathetic nerve
- Adrenergic alpha-Antagonists
- Adrenergic beta-Agonists
- Adrenergic beta-Antagonists
- Animals
- Arginine
- Blood Pressure
- Electric Stimulation
- Enzyme Inhibitors
- Isoproterenol
- NG-Nitroarginine Methyl Ester
- Rats
- Rats
- Sprague-Dawley
- Saliva
- Salivary Proteins
- Sympathetic Nervous System
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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