Nitric oxide-dependent protein synthesis in parotid and submandibular glands of anaesthetized rats upon sympathetic stimulation or isoprenaline administration

• In anaesthetized female rats, the β-adrenoceptor agonist isoprenaline was intravenously infused (20 μg kg-1 min-1) for 30 min or the ascending cervical sympathetic nerve trunk was intermittently stimulated (50 Hz, 1 s every tenth second) on one side for 30 min. The incorporation of [3H]leucine into trichloroacetic acid (TCA)-insoluble material was used as an index of protein synthesis. In response to isoprenaline, the [3H]leucine incorporation increased by 79% in the parotid glands and by 82% in the submandibular glands. The neuronal type NO-synthase inhibitor N-PLA, reduced (P < 0.001) this response to 26% and 20%, respectively. Sympathetic stimulation under α-adrenoceptor blockade increased the [3H]leucine incorporation by 192% in the parotid glands and by 35% in the submandibular glands. N-PLA reduced the corresponding percentage figures to 86% (P < 0.01) and 8% (P < 0.05). When tested in the parotid glands, the non-selective NO-synthase inhibitor L-NAME reduced (P < 0.01) the nerve-evoked response to 91%. The increase in [3H]leucine incorporation in response to sympathetic stimulation under β-adrenoceptor blockade was not affected by N-PLA in the parotid (139% versus 144%) and submandibular glands (39% versus 34%). In non-stimulated glands, the [3H]leucine incorporation was not influenced by the NO-synthase inhibitors. In conclusion, β-adrenoceptor mediated salivary gland protein synthesis is largely dependent on NO generation by neuronal type NO-synthase, most likely of parenchymal origin. 

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Physiology (hsv//eng)

Nyckelord

beta adrenergic receptor blocking agent

isoprenaline

leucine

n(g) nitroarginine methyl ester

nitric oxide

nitric oxide synthase

nitric oxide synthase inhibitor

trichloroacetic acid

adrenergic stimulation

animal tissue

article

beta adrenergic receptor blocking

controlled study

enzyme inhibition

evoked response

female

nerve stimulation

nonhuman

parotid gland

protein synthesis

rat

statistical analysis

statistical significance

submandibular gland

sympathetic nerve

Adrenergic alpha-Antagonists

Adrenergic beta-Agonists

Adrenergic beta-Antagonists

Animals

Arginine

Blood Pressure

Electric Stimulation

Enzyme Inhibitors

Isoproterenol

NG-Nitroarginine Methyl Ester

Rats

Rats

Sprague-Dawley

Saliva

Salivary Proteins

Sympathetic Nervous System

Publikations- och innehållstyp

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art (ämneskategori)