Sökning: WFRF:(Andreasson Sven) > Femtosecond X-ray p...
Fältnamn | Indikatorer | Metadata |
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000 | 08421naa a2201729 4500 | |
001 | oai:gup.ub.gu.se/158022 | |
003 | SwePub | |
008 | 240528s2011 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-146068 | |
009 | oai:slubar.slu.se:33574 | |
024 | 7 | a https://gup.ub.gu.se/publication/1580222 URI |
024 | 7 | a https://doi.org/10.1038/nature097502 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1460682 URI |
024 | 7 | a https://res.slu.se/id/publ/335742 URI |
040 | a (SwePub)gud (SwePub)uud (SwePub)slu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Chapman, Henry N4 aut |
245 | 1 0 | a Femtosecond X-ray protein nanocrystallography. |
264 | c 2011-02-02 | |
264 | 1 | b Springer Science and Business Media LLC,c 2011 |
338 | a electronic2 rdacarrier | |
520 | a X-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the necessary increase in X-ray dose to record data from crystals that are too small leads to extensive damage before a diffraction signal can be recorded. It is particularly challenging to obtain large, well-diffracting crystals of membrane proteins, for which fewer than 300 unique structures have been determined despite their importance in all living cells. Here we present a method for structure determination where single-crystal X-ray diffraction 'snapshots' are collected from a fully hydrated stream of nanocrystals using femtosecond pulses from a hard-X-ray free-electron laser, the Linac Coherent Light Source. We prove this concept with nanocrystals of photosystem I, one of the largest membrane protein complexes. More than 3,000,000 diffraction patterns were collected in this study, and a three-dimensional data set was assembled from individual photosystem I nanocrystals (∼200nm to 2μm in size). We mitigate the problem of radiation damage in crystallography by using pulses briefer than the timescale of most damage processes. This offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Andra medicinska och farmaceutiska grundvetenskaper0 (SwePub)301992 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Other Basic Medicine0 (SwePub)301992 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Fysiologi0 (SwePub)301062 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Physiology0 (SwePub)301062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Fysikx Atom- och molekylfysik och optik0 (SwePub)103022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Physical Sciencesx Atom and Molecular Physics and Optics0 (SwePub)103022 hsv//eng |
653 | a Crystallography | |
653 | a X-Ray | |
653 | a instrumentation | |
653 | a methods | |
653 | a Lasers | |
653 | a Models | |
653 | a Molecular | |
653 | a Nanoparticles | |
653 | a chemistry | |
653 | a Nanotechnology | |
653 | a instrumentation | |
653 | a methods | |
653 | a Photosystem I Protein Complex | |
653 | a chemistry | |
653 | a Protein Conformation | |
653 | a Time Factors | |
653 | a X-Rays | |
653 | a Biology | |
700 | 1 | a Fromme, Petra4 aut |
700 | 1 | a Barty, Anton4 aut |
700 | 1 | a White, Thomas A4 aut |
700 | 1 | a Kirian, Richard A4 aut |
700 | 1 | a Aquila, Andrew4 aut |
700 | 1 | a Hunter, Mark S4 aut |
700 | 1 | a Schulz, Joachim4 aut |
700 | 1 | a DePonte, Daniel P4 aut |
700 | 1 | a Weierstall, Uwe4 aut |
700 | 1 | a Doak, R Bruce4 aut |
700 | 1 | a Maia, Filipe R. N. C.u Gothenburg University,Göteborgs universitet,Uppsala universitet,Molekylär biofysik,Institutionen för kemi,Department of Chemistry4 aut0 (Swepub:gu)xneuri |
700 | 1 | a Martin, Andrew V4 aut |
700 | 1 | a Schlichting, Ilme4 aut |
700 | 1 | a Lomb, Lukas4 aut |
700 | 1 | a Coppola, Nicola4 aut |
700 | 1 | a Shoeman, Robert L4 aut |
700 | 1 | a Epp, Sascha W4 aut |
700 | 1 | a Hartmann, Robert4 aut |
700 | 1 | a Rolles, Daniel4 aut |
700 | 1 | a Rudenko, Artem4 aut |
700 | 1 | a Foucar, Lutz4 aut |
700 | 1 | a Kimmel, Nils4 aut |
700 | 1 | a Weidenspointner, Georg4 aut |
700 | 1 | a Holl, Peter4 aut |
700 | 1 | a Liang, Mengning4 aut |
700 | 1 | a Barthelmess, Miriam4 aut |
700 | 1 | a Caleman, Carl4 aut |
700 | 1 | a Boutet, Sébastien4 aut |
700 | 1 | a Bogan, Michael J4 aut |
700 | 1 | a Krzywinski, Jacek4 aut |
700 | 1 | a Bostedt, Christoph4 aut |
700 | 1 | a Bajt, Saša4 aut |
700 | 1 | a Gumprecht, Lars4 aut |
700 | 1 | a Rudek, Benedikt4 aut |
700 | 1 | a Erk, Benjamin4 aut |
700 | 1 | a Schmidt, Carlo4 aut |
700 | 1 | a Hömke, André4 aut |
700 | 1 | a Reich, Christian4 aut |
700 | 1 | a Pietschner, Daniel4 aut |
700 | 1 | a Strüder, Lothar4 aut |
700 | 1 | a Hauser, Günter4 aut |
700 | 1 | a Gorke, Hubert4 aut |
700 | 1 | a Ullrich, Joachim4 aut |
700 | 1 | a Herrmann, Sven4 aut |
700 | 1 | a Schaller, Gerhard4 aut |
700 | 1 | a Schopper, Florian4 aut |
700 | 1 | a Soltau, Heike4 aut |
700 | 1 | a Kühnel, Kai-Uwe4 aut |
700 | 1 | a Seibert, M. Marvinu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)masei255 |
700 | 1 | a Bozek, John D4 aut |
700 | 1 | a Hau-Riege, Stefan P4 aut |
700 | 1 | a Frank, Matthias4 aut |
700 | 1 | a Hampton, Christina Y4 aut |
700 | 1 | a Sierra, Raymond G4 aut |
700 | 1 | a Starodub, Dmitri4 aut |
700 | 1 | a Williams, Garth J4 aut |
700 | 1 | a Hajdu, Janosu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)janohajd |
700 | 1 | a Timneanu, Nicusoru Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)nti19847 |
700 | 1 | a Seibert, M Marvin4 aut |
700 | 1 | a Andreasson, Jakobu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)jakan998 |
700 | 1 | a Rocker, Andreau Uppsala universitet,Molekylär biofysik4 aut |
700 | 1 | a Jönsson, Olofu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)olojo993 |
700 | 1 | a Svenda, Martinu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)msv31659 |
700 | 1 | a Stern, Stephan4 aut |
700 | 1 | a Nass, Karol4 aut |
700 | 1 | a Andritschke, Robert4 aut |
700 | 1 | a Schröter, Claus-Dieter4 aut |
700 | 1 | a Krasniqi, Faton4 aut |
700 | 1 | a Bott, Mario4 aut |
700 | 1 | a Schmidt, Kevin E4 aut |
700 | 1 | a Wang, Xiaoyu4 aut |
700 | 1 | a Grotjohann, Ingo4 aut |
700 | 1 | a Holton, James M4 aut |
700 | 1 | a Barends, Thomas R M4 aut |
700 | 1 | a Neutze, Richard,d 1969u Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry4 aut0 (Swepub:gu)xneuri |
700 | 1 | a Marchesini, Stefano4 aut |
700 | 1 | a Fromme, Raimund4 aut |
700 | 1 | a Schorb, Sebastian4 aut |
700 | 1 | a Rupp, Daniela4 aut |
700 | 1 | a Adolph, Marcus4 aut |
700 | 1 | a Gorkhover, Tais4 aut |
700 | 1 | a Andersson, Ingeru Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology4 aut0 (Swepub:slu)47211 |
700 | 1 | a Hirsemann, Helmut4 aut |
700 | 1 | a Potdevin, Guillaume4 aut |
700 | 1 | a Graafsma, Heinz4 aut |
700 | 1 | a Nilsson, Björn4 aut |
700 | 1 | a Spence, John C H4 aut |
710 | 2 | a Uppsala universitetb Molekylär biofysik4 org |
710 | 2 | a Sveriges lantbruksuniversitet |
773 | 0 | t Natured : Springer Science and Business Media LLCg 470:7332, s. 73-7q 470:7332<73-7x 1476-4687x 0028-0836 |
856 | 4 | u https://gup.ub.gu.se/publication/158022x primaryx freey FULLTEXT |
856 | 4 | u https://bib-pubdb1.desy.de/record/87161/files/Chapman-merged.pdf |
856 | 4 8 | u https://gup.ub.gu.se/publication/158022 |
856 | 4 8 | u https://doi.org/10.1038/nature09750 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-146068 |
856 | 4 8 | u https://res.slu.se/id/publ/33574 |
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