Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells

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Fältnamn	Indikatorer	Metadata
000		04952naa a2200469 4500
001		oai:gup.ub.gu.se/305575
003		SwePub
008		240528s2021 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:prod.swepub.kib.ki.se:146720862
024	7	a https://gup.ub.gu.se/publication/3055752 URI
024	7	a https://doi.org/10.3389/fimmu.2021.6775502 DOI
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1467208622 URI
040		a (SwePub)gud (SwePub)ki
041		a eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Ramos-Ramírez, Patriciau Gothenburg University,Göteborgs universitet,Krefting Research Centre,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition4 aut0 (Swepub:gu)xrampa
245	1 0	a Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells
264		c 2021-05-18
264	1	b Frontiers Media SA,c 2021
520		a Background Adiponectin is an important immunomodulatory mediator in inflammatory conditions. While we previously showed that adiponectin receptor 1 (AdipoR1) is expressed in murine regulatory T cells (Tregs), its expression in human Tregs remain unknown. Here, we examined the expression of AdipoR1 in human Tregs and whether its ligand, globular adiponectin (gAd) affects the Treg ability to secrete IL-10 and the role of Type 2 (T2) inflammation in such process. Methods Human Tregs from peripheral blood were analyzed by flow cytometry for AdipoR1, Helios and IL-10 expression. CD4(+) T cells enriched from peripheral blood mononuclear cells (PBMCs) were cultured in the presence or the absence of gAd or the chemical adiponectin receptor agonist, AdipoRon, or in a T2 cytokine milieu. Flow cytometry was then used to assess intracellular IL-10, IL-10 secreting cells, FOXP3 and Helios expression, and phosphorylated p38 MAP kinase (MAPK). IL-10 levels in CD4(+) T cell supernatants were quantified by ELISA. Results We found that a subset of human Tregs expressed AdipoR1. Importantly, more Helios(-) cells expressed AdipoR1 than Helios(+) cells. Likewise, there was a higher frequency of IL-10(+) cells within Helios(-) AdipoR1(+) Tregs compared to Helios(+) AdipoR1(+) Tregs. In contrast, the IL-10 mean fluorescence intensity (MFI) was higher in Helios(+) AdipoR1(+) Tregs compared to Helios(-)AdipoR1(+) Tregs. When human CD4(+) T cells were treated with gAd or AdipoRon, a significant increase in IL-10 secretion, FOXP3 expression, and p38 MAPK phosphorylation was observed in Helios(-) AdipoR1(+) Tregs. Interestingly, gAd under T2 cytokine milieu significantly increased the intracellular levels of IL-10, mainly in Helios(+) AdipoR1(+) Tregs, and IL-10 levels in supernatants of CD4(+) T cells. Conclusions Collectively, our findings suggest that adiponectin/AdipoR1 axis promotes IL-10 release by Tregs, mainly in Helios(-) Tregs, and the effect was amplified by T2 inflammation in Helios(+) Tregs.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng
653		a adiponectin receptor 1
653		a regulatory T cells
653		a interleukin-10
653		a adiponectin
653		a type 2 inflammation
653		a homeostasis
653		a receptors
653		a Immunology
700	1	a Malmhäll, Carina,d 1959u Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Krefting Research Centre,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition4 aut0 (Swepub:gu)xmalmc
700	1	a Tliba, O.4 aut
700	1	a Rådinger, Madeleine,d 1967u Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Krefting Research Centre,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition4 aut0 (Swepub:gu)xradim
700	1	a Bossios, Apostolos,d 1969u Karolinska Institutet,Gothenburg University,Göteborgs universitet,Krefting Research Centre,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition4 aut0 (Swepub:gu)xbosap
710	2	a Göteborgs universitetb Krefting Research Centre4 org
773	0	t Frontiers in Immunologyd : Frontiers Media SAg 12q 12x 1664-3224
856	4	u https://www.frontiersin.org/articles/10.3389/fimmu.2021.677550/pdf
856	4 8	u https://gup.ub.gu.se/publication/305575
856	4 8	u https://doi.org/10.3389/fimmu.2021.677550
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:146720862

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