Ultrastructure and Mechanical Properties of Populus Wood with Reduced Lignin Content Caused by Transgenic Down-Regulation of Cinnamate 4-Hydroxylase

QC 20101129
cited By 35
Several key enzymes in lignin biosynthesis of Populus have been down-regulated by transgenie approaches to investigate their role in wood lignification and to explore their potential for lignin modification. Cinnamate 4-hydroxylase is an enzyme in the early phenylpropanoid pathway that has not yet been functionally analyzed in Populus. This study shows that down-regulation of cinnamate 4-hydroxylase reduced Klason lignin content by 30% with no significant change in syringyl to guaiacyl ratio. The lignin reduction resulted in ultrastructural differences of the wood and a 10% decrease in wood density. Mechanical properties investigated by tensile tests and dynamic mechanical analysis showed a decrease in stiffness, which could be explained by the lower density. The study demonstrates that a large modification in lignin content only has minor influences on tensile properties of wood in its axial direction and highlights the usefulness of wood modified beyond its natural variation by transgene technology in exploring the impact of wood biopolymer composition and ultrastructure on its material properties.

NATURVETENSKAP Kemi hsv//swe
NATURAL SCIENCES Chemical Sciences hsv//eng
TEKNIK OCH TEKNOLOGIER Materialteknik Pappers-, massa- och fiberteknik hsv//swe
ENGINEERING AND TECHNOLOGY Materials Engineering Paper, Pulp and Fiber Technology hsv//eng
LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Skogsvetenskap hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Forest Science hsv//eng
Axial direction
Cinnamate 4-hydroxylase
Down-regulation
Guaiacyl
Key enzymes
Klason lignin
Lignin biosynthesis
Lignin contents
Lignin modifications
Lower density
Material property
Natural variation
Phenylpropanoid pathways
Populus
Tensile tests
Transgene
Transgenic approaches
Transgenics
Wood density
Biochemistry
Density (specific gravity)
Dynamic analysis
Dynamic mechanical analysis
Enzymes
Lignin
Plants (botany)
Stiffness
Tensile testing
Wood
Chemistry
Kemi

Olsson, Anne-MariRISE,Innventia (författare)
Zhang, BoDepartment of Biomaterials, Max Planck Institute of Colloids and Interfaces (författare)
Gerber, LorenzSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)50493 (författare)
Kumar, ManojSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)52097 (författare)
Berglund, Lars A.KTH,Biokompositer(Swepub:kth)u1wqz3r1 (författare)
Burgert, IngoDepartment of Biomaterials, Max Planck Institute of Colloids and Interfaces (författare)
Sundberg, BjörnSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)46876 (författare)
Salmen, LennartRISE,Innventia(Swepub:ri)lennart.salmen@ri.se (författare)
KTHBiokompositer (creator_code:org_t)
Sveriges lantbruksuniversitet

Ingår i:Biomacromolecules: American Chemical Society (ACS)11:9, s. 2359-23651525-77971526-4602

Av författaren/redakt...: Bjurhager, Ingel ...; Olsson, Anne-Mar ...; Zhang, Bo; Gerber, Lorenz; Kumar, Manoj; Berglund, Lars A ...; visa fler...; Burgert, Ingo; Sundberg, Björn; Salmen, Lennart; visa färre...

Om ämnet

TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Materialteknik; och Pappers massa oc ...

LANTBRUKSVETENSKAPER: LANTBRUKSVETENSK ...; och Lantbruksvetensk ...; och Skogsvetenskap

Av lärosätet: Kungliga Tekniska Högskolan; RISE; Sveriges Lantbruksuniversitet

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