L773:0196 3635
Sökning: L773:0196 3635 > Distribution and ti...
- Bjartell, AndersLund University,Lunds universitet,Urologisk cancerforskning, Malmö,Forskargrupper vid Lunds universitet,Urological cancer, Malmö,Lund University Research Groups (författare)
Distribution and tissue expression of semenogelin I and II in man as demonstrated by in situ hybridization and immunocytochemistry
- Artikel/kapitelEngelska1996
Förlag, utgivningsår, omfång ...
- 1996
Nummerbeteckningar
- LIBRIS-ID:oai:lup.lub.lu.se:c975ca2d-e2a4-4e85-a813-f1194c363a8c
- https://lup.lub.lu.se/record/3965237URI
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- Språk:engelska
- Sammanfattning på:engelska
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- Semenogelin I and II (Sgl, Sgll) are two separate gene products of chromosome 20 with extensive (80%) identity in primary structure. They are mainly responsible for immediate gel formation of freshly ejaculated semen. Degradation of Sgl and Sgll is due to the proteolytic action of prostate-specific antigen (PSA); it results within 5-15 minutes in liquefaction of semen and release of progressively motile spermatozoa. By means of cDNA cloning and Northern blots, Sgl and Sgll transcripts have previously been shown to be abundant in human seminal vesicles, but Sgll alone is suggested to be expressed at low levels in the epididymis. To characterize the expression and tissue distribution of Sgl and Sgll in greater detail, we produced monoclonal immunoglobulin Gs (lgGs for immunocytochemistry (lCC) and specific [35S]-, digoxigenin-, or alkaline phosphatase-labeled 30-mer antisense probes to Sgl and Sgll for in situ hybridization (lSH). Immunocytochemical staining for both Sgl and Sgll, and lSH detection of both Sgl and Sgll transcripts, were demonstrated in the cytoplasm of seminal vesicle epithelium. lSH showed Sgll alone to be expressed in the epithelium of the epididymal cauda. Neither lCC nor lSH yielded any evidence of Sgl or Sgll expression in caput or corpus epithelium or in any stromal cells of the epididymis. Consistent with our previous findings using polyclonal lgG, monoclonal anti-Sgll Sgll lgGs identified epitopes on the posterior head, midpiece, and tail of ejaculated spermatozoa. Spermatozoa in the epididymal cauda were also immunoreactive, but those in the caput or corpus region of the epididymis as well as those in the testis were negative. As shown by lCC, neither Sgl nor Sgll were expressed in the testis, the prostate, the female genital tract, or other normal human tissue specimens. Although the significance of Sg attachment to epididymal and ejaculated spermatozoa remains to be established, monoclonal anti-Sg lgG might prove useful in establishing the origin of seminal vesicle tissue components in prostate core biopsies or other biopsy specimens.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Läkemedelskemi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Medicinal Chemistry hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Urologi och njurmedicin hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Urology and Nephrology hsv//eng
- Oligonucleotide Probes
- Middle Aged
- Mice
- Male
- In Situ Hybridization
- Immunohistochemistry
- Humans
- Gonadal Steroid Hormones/analysis/*genetics/*immunology
- Gene Expression/physiology
- Epididymis/cytology
- Digoxigenin
- Southern
- Blotting
- Antibody Specificity
- Monoclonal
- Antibodies
- Animals
- 80 and over
- Aged
- Prostate/cytology
- Protein Precursors/analysis/*genetics/*immunology
- RNA
- Messenger/analysis
- Rabbits
- Research Support
- Non-U.S. Gov't
- *Seminal Plasma Proteins
- *Seminal Vesicle Secretory Proteins
- Seminal Vesicles/cytology
- Spermatozoa/chemistry/physiology
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Malm, JohanLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-jma (författare)
- Möller, C. (författare)
- Gunnarsson, M. (författare)
- Lundwall, ÅkeLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-alu (författare)
- Lilja, HansLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-hli (författare)
- Urologisk cancerforskning, MalmöForskargrupper vid Lunds universitet (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Journal of Andrology17, s. 17-260196-3635
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- Bjartell, Anders
- Malm, Johan
- Möller, C.
- Gunnarsson, M.
- Lundwall, Åke
- Lilja, Hans
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