L773:0271 3683 OR L773:1460 2202
Sökning: L773:0271 3683 OR L773:1460 2202 > Xenobiotic- and Ser...
Xenobiotic- and Serum-Free Culture of Oral Mucosal Epithelial Cells on Contact Lenses
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- Björkblom, Benny (författare)
- Umeå universitet,Kemiska institutionen
- Eidet, Jon R. (författare)
- Utheim, Tor P. (författare)
- visa fler...
- Ulltveit-Moe, Harald F. (författare)
- Raeder, Sten (författare)
- visa färre...
- (creator_code:org_t)
- 2015-02-06
- 2016
- Engelska.
- Ingår i: Current Eye Research. - : Informa UK Limited. - 0271-3683 .- 1460-2202. ; 41:1, s. 20-27
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Purpose/aim: Cultured autologous oral mucosal epithelial cells (OMECs) have proven useful in the treatment of ocular surface disorders. This study is the first to investigate the potential of expanding OMEC in a xenobiotic- and serum-free medium using therapeutic contact lenses (CLs) as a substrate and carrier.Materials and methods: Porcine OMEC were seeded on laminin-coated lotrafilcon A therapeutic CLs with the density of 8x10(4)cells/lens and cultured in a defined serum and xenobiotic-free medium. Confocal immunofluorescence microscopy was used to analyze the following: (1) cellular morphology by using rhodamine-phalloidin staining of F-actin, (2) phenotype by applying antibodies against the progenitor cell marker p63 and the putative stem cell marker ABCG2 and (3) cell viability by using propidium iodide and Hoechst 33342 dual staining.Results: Porcine OMEC attached well to the CLs, and cell-to-cell contacts were evident. After three days in culture, the OMEC displayed a confluent monolayer with uniform cobblestone morphology, whereas stratified cultures with 2-3 layers were formed after six days. No significant difference in expression of p63 was observed after three-day culture (79.414.8%) compared with six-day culture (60.3 +/- 18.9%). ABCG2 expression in the basal cell layer was 6.3 +/- 1.0% and 4.8 +/- 1.8% after three- and six-day culture, respectively. The basal layer viability of cultured OMECs was 99.3 +/- 0.2% and 82.8 +/- 1.1% after three and six days culture, respectively.Conclusions: The use of therapeutic CLs has potential as a substrate and carrier for OMEC cultured in a xenobiotic- and serum-free culture system.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
Nyckelord
- Cell culture
- oral mucosa
- contact lens
- epithelial cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
- Björkblom, Benny
- Eidet, Jon R.
- Utheim, Tor P.
- Ulltveit-Moe, Ha ...
- Raeder, Sten
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Oftalmologi
- Artiklar i publikationen
- Current Eye Rese ...
- Av lärosätet
- Umeå universitet
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