L773:1878 5492 OR L773:0966 3274
Search: L773:1878 5492 OR L773:0966 3274 > (2010-2014) > Isolation, expansio...
- 4 of 11
- Previous record
- Next record
- To hitlist
- Berglund, DavidUppsala universitet,Transplantationskirurgi,Klinisk immunologi,Gunnar Tufveson, Olle Korsgren (author)
Isolation, expansion and functional assessment of CD4+CD25+FoxP3+ regulatory T cells and Tr1 cells from uremic patients awaiting kidney transplantation
- Article/chapterEnglish2012
Publisher, publication year, extent ...
- Elsevier BV,2012
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-170517
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-170517URI
- https://doi.org/10.1016/j.trim.2011.09.003DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Background: The immunosuppressive properties of regulatory T cells have emerged as an attractive tool for the development of immunotherapies in various disease contexts, e.g. to treat transplantation induced immune reactions. This paper focuses on the process of obtaining and functionally characterizing CD4+CD25+FoxP3+ regulatory T cells and Tr1 cells from uremic patients awaiting kidney transplantation. Methods: From October 2010 to March 2011 uremic patients awaiting living donor kidney transplantation, and their corresponding kidney donors, were enrolled in the study. A total of seven pairs were included. Isolation of CD4+CD25+FoxP3+ regulatory T cells was performed by magnetic activated cell sorting of peripheral blood mononuclear cells obtained from the uremic patients. Donor specific Tr1 cells were differentiated by repetitive stimulation of immature CD4+ T cells with immature dendritic cells, with the T cells coming from the future kidney recipients and the dendritic cells from the corresponding kidney donors. Cells were then expanded and functionally characterized by the one-way mixed leukocyte reaction and assessment of IL-10 production. Phenotypic analysis was performed by flow cytometry. Results: The fraction of CD4+CD25+FoxP3+ regulatory T cells after expansion varied from 39.1 to 50.4% and the cells retained their ability to substantially suppress the mixed leukocyte reaction in all but one patient (3.8–19.2% of the baseline stimulated leukocyte activity, p<0.05). Tr1 cells were successfully differentiated from all but one patient and produced high levels of IL-10 when stimulated with immature dendritic cells (1,275–11,038% of the baseline IL-10 secretion, pb0.05). Conclusion: It is practically feasible to obtain and subsequently expand CD4+CD25+FoxP3+ regulatory T cells and Tr1 cells from uremic patients without loss of function as assessed by in vitro analyses. This forms a base for adoptive regulatory T cell therapy in the setting of living donor kidney transplantation.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
- Korsgren, OlleUppsala universitet,Klinisk immunologi(Swepub:uu)ollekors (author)
- Lorant, TomasUppsala universitet,Transplantationskirurgi(Swepub:uu)tomalora (author)
- Schneider, KarinUppsala universitet,Klinisk immunologi(Swepub:uu)karischn (author)
- Tufveson, GunnarUppsala universitet,Transplantationskirurgi(Swepub:uu)gutuf839 (author)
- Carlsson, BjörnUppsala universitet,Klinisk immunologi (author)
- Uppsala universitetTransplantationskirurgi (creator_code:org_t)
Related titles
- In:Transplant Immunology: Elsevier BV26:1, s. 27-330966-32741878-5492
Internet link
Find in a library
- Transplant Immunology (Search for host publication in LIBRIS)
To the university's database
- 4 of 11
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Berglund, David
- Korsgren, Olle
- Lorant, Tomas
- Schneider, Karin
- Tufveson, Gunnar
- Carlsson, Björn
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Immunology in th ...
- Articles in the publication
- Transplant Immun ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
