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Capacitive Sacchari...
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Bergdahl, Gizem ErtürkLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups,CapSenze Biosystems AB
(författare)
Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
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2018-10-28
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Springer Science and Business Media LLC,2019
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:9f128505-60c2-4223-b50d-d40bf90a1584
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https://lup.lub.lu.se/record/9f128505-60c2-4223-b50d-d40bf90a1584URI
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https://doi.org/10.1007/s12010-018-2911-3DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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A capacitive sensor for saccharide detection is described in this study. The detection is based on selective interaction between diols and aminophenylboronic acid (APBA) immobilized on a gold electrode. Glucose, fructose, and dextran (MW: 40 kDa) were tested with the system over wide concentration ranges (1.0 x 10−8 M - 1.0 x 10−3 M for glucose, 1.0 x 10−8 M - 1.0 x 10−2 M for fructose and 1.0 x 10−10 M - 1.0 x 10−5 M for dextran). The limits of detection (LODs) were 0.8 nM for glucose, 0.6 nM for fructose, and 13 pM for dextran. These data were comparable to the others reported previously. In order to demonstrate glycoprotein detection with the same sensor, human immunoglobulin G (IgG) as well as horseradish peroxidase were used as model analytes. The sensor responded to IgG in the concentration range of 1.0 x 10−13 M - 1.0 x 10−7 M with a LOD value of 16 fM. The performance of the assay of peroxidase was compared to a spectrophotometric assay by determining the enzymatic activity of a captured analyte. The results showed that the method might be useful for label-free, fast, and sensitive detection of saccharides as well as glycoproteins over a wide concentration range.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Hedström, MartinLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze Biosystems AB(Swepub:lu)biot-mhe
(författare)
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Mattiasson, BoLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze Biosystems AB(Swepub:lu)biot-bma
(författare)
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BioteknikCentrum för tillämpade biovetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Applied Biochemistry and Biotechnology: Springer Science and Business Media LLC188:1, s. 124-1370273-22891559-0291
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