WFRF:(Cascante M)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03793naa a2200541 4500 | |
| 001 | oai:gup.ub.gu.se/50345 | |
| 003 | SwePub | |
| 008 | 240528s2006 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://gup.ub.gu.se/publication/503452 URI |
| 040 | a (SwePub)gu | |
| 041 | a eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Borén, Jan,d 1963u Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberg Laboratory,Institute of Medicine, Department of Molecular and Clinical Medicine4 aut0 (Swepub:gu)xborej |
| 245 | 1 0 | a In situ localization of transketolase activity in epithelial cells of different rat tissues and subcellularly in liver parenchymal cells |
| 264 | 1 | c 2006 |
| 520 | a Metabolic mapping of enzyme activities (enzyme histochemistry) is an important tool to understand (patho)physiological functions of enzymes. A new enzyme histochemical method has been developed to detect transketolase activity in situ in various rat tissues and its ultrastructural localization in individual cells. In situ detection of transketolase is important because this multifunctional enzyme has been related with diseases such as cancer, diabetes, Alzheimer's disease, and Wernicke-Korsakoff's syndrome. The proposed method is based on the tetrazolium salt method applied to unfixed cryostat sections in the presence of polyvinyl alcohol. The method appeared to be specific for transketolase activity when the proper control reaction is performed and showed a linear increase of the amount of final reaction product with incubation time. Transketolase activity was studied in liver, small intestine, trachea, tongue, kidney, adrenal gland, and eye. Activity was found in liver parenchyma, epithelium of small intestine, trachea, tongue, proximal tubules of kidney and cornea, and ganglion cells in medulla of adrenal gland. To demonstrate transketolase activity ultrastructurally in liver parenchymal cells, the cupper iron method was used. It was shown that transketolase activity was present in peroxisomes and at membranes of granular endoplasmic reticulum. This ultrastructural localization is similar to that of glucose-6-phosphate dehydrogenase activity, suggesting activity of the pentose phosphate pathway at these sites. It is concluded that the method developed for in situ localization of transketolase activity for light and electron microscopy is specific and allows further investigation of the role of transketolase in (proliferation of) cancer cells and other pathophysiological processes. | |
| 653 | a Animals | |
| 653 | a Cornea/enzymology | |
| 653 | a Endoplasmic Reticulum/enzymology/ultrastructure | |
| 653 | a Epithelial Cells/*enzymology | |
| 653 | a Intestine | |
| 653 | a Small/enzymology | |
| 653 | a Intracellular Membranes/enzymology | |
| 653 | a Kidney Tubules | |
| 653 | a Proximal/enzymology | |
| 653 | a Liver/*enzymology/ultrastructure | |
| 653 | a Male | |
| 653 | a Neurons/metabolism | |
| 653 | a Organ Specificity | |
| 653 | a Peroxisomes/enzymology | |
| 653 | a Rats | |
| 653 | a Rats | |
| 653 | a Wistar | |
| 653 | a Tongue/enzymology | |
| 653 | a Trachea/enzymology | |
| 653 | a Transketolase/*metabolism | |
| 700 | 1 | a Ramos-Montoya, A.4 aut |
| 700 | 1 | a Bosch, K. S.4 aut |
| 700 | 1 | a Vreeling, H.4 aut |
| 700 | 1 | a Jonker, A.4 aut |
| 700 | 1 | a Centelles, J. J.4 aut |
| 700 | 1 | a Cascante, M.4 aut |
| 700 | 1 | a Frederiks, W. M.4 aut |
| 710 | 2 | a Göteborgs universitetb Wallenberglaboratoriet4 org |
| 773 | 0 | t J Histochem Cytochemg 54:2, s. 191-9q 54:2<191-9x 0022-1554 |
| 856 | 4 8 | u https://gup.ub.gu.se/publication/50345 |
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- Borén, Jan, 1963
- Ramos-Montoya, A ...
- Bosch, K. S.
- Vreeling, H.
- Jonker, A.
- Centelles, J. J.
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- Cascante, M.
- Frederiks, W. M.
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- J Histochem Cyto ...
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- Göteborgs universitet
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