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A multi-omics appro...
A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees
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- Obudulu, Ogonna (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Umeå universitet,Kemiska institutionen,Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183 Umeå, Sweden,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Umeå University
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- Mähler, Niklas (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Faculty of Chemistry, Biotechnology and Food Science, Norwegian, University of Life Sciences, 1432 Ås, Norway
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- Skotare, Tomas (författare)
- Umeå universitet,Kemiska institutionen
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- Bygdell, Joakim (författare)
- Umeå universitet,Kemiska institutionen
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Abreu, Ilka N. (författare)
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- Ahnlund, Maria (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Latha Gandla, Madhavi (författare)
- Umeå universitet,Kemiska institutionen
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- Petterle, Anna (författare)
- Umeå universitet,Institutionen för fysiologisk botanik
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- Moritz, Thomas (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Hvidsten, Torgeir R. (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Faculty of Chemistry, Biotechnology and Food Science, Norwegian, University of Life Sciences, 1432 Ås, Norway
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- Jönsson, Leif J. (författare)
- Umeå universitet,Kemiska institutionen
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- Wingsle, Gunnar (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Trygg, Johan (författare)
- Umeå universitet,Kemiska institutionen
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- Tuominen, Hannele (författare)
- Umeå universitet,Institutionen för fysiologisk botanik
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(creator_code:org_t)
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- 2018-01-03
- 2018
- Engelska.
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Ingår i: BMC Genomics. - : Springer Publishing Company. - 1471-2164. ; 19
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://bmcgenomics....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- Background: Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved 32–38 kDa proteins that are involved in membrane trafficking. A systems approach was taken to elucidate function of SCAMPs in wood formation of Populus trees. Phenotypic and multi-omics analyses were performed in woody tissues of transgenic Populus trees carrying an RNAi construct for Populus tremula x tremuloides SCAMP3 (PttSCAMP3;Potri.019G104000).Results: The woody tissues of the transgenic trees displayed increased amounts of both polysaccharides and lignin oligomers, indicating increased deposition of both the carbohydrate and lignin components of the secondary cell walls. This coincided with a tendency towards increased wood density as well as significantly increased thickness of the suberized cork in the transgenic lines. Multivariate OnPLS (orthogonal projections to latent structures) modeling of five different omics datasets (the transcriptome, proteome, GC-MS metabolome, LC-MS metabolome and pyrolysis-GC/MS metabolome) collected from the secondary xylem tissues of the stem revealed systemic variation in the different variables in the transgenic lines, including changes that correlated with the changes in the secondary cell wall composition. The OnPLS model also identified a rather large number of proteins that were more abundant in the transgenic lines than in the wild type. Several of these were related to secretion and/or endocytosis as well as both primary and secondary cell wall biosynthesis.Conclusions: Populus SCAMP proteins were shown to influence accumulation of secondary cell wall components, including polysaccharides and phenolic compounds, in the woody tissues of Populus tree stems. Our multi-omics analyses combined with the OnPLS modelling suggest that this function is mediated by changes in membrane trafficking to fine-tune the abundance of cell wall precursors and/or proteins involved in cell wall biosynthesis and transport. The data provides a multi-level source of information for future studies on the function of the SCAMP proteins in plant stem tissues.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Skogsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Forest Science (hsv//eng)
Nyckelord
- Secretory Carrier-Associated Membrane Protein (SCAMP)
- Populus
- Wood chemistry
- Wood density
- Biomass
- Bioprocessing
- Cork
- Multi-omics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Obudulu, Ogonna
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Mähler, Niklas
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Skotare, Tomas
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Bygdell, Joakim
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Abreu, Ilka N.
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Ahnlund, Maria
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visa fler...
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Latha Gandla, Ma ...
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Petterle, Anna
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Moritz, Thomas
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Hvidsten, Torgei ...
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Jönsson, Leif J.
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Wingsle, Gunnar
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Trygg, Johan
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Tuominen, Hannel ...
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visa färre...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
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- LANTBRUKSVETENSKAPER
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LANTBRUKSVETENSK ...
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och Lantbruksvetensk ...
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och Skogsvetenskap
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BMC Genomics
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Umeå universitet
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