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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
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001oai:DiVA.org:uu-435198
003SwePub
008210226s2021 | |||||||||||000 ||eng|
009oai:slubar.slu.se:109965
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4351982 URI
0247 a https://doi.org/10.1186/s12936-020-03574-12 DOI
0247 a https://res.slu.se/id/publ/1099652 URI
040 a (SwePub)uud (SwePub)slu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Rocha, Elerson Matosu Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut
2451 0a Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential
264 c 2021-01-13
264 1b BioMed Central (BMC),c 2021
338 a electronic2 rdacarrier
500 a Correction in: Malaria Journal, vol. 20, issue 1, article number 83.DOI: 10.1186/s12936-021-03609-1
520 a Background: Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans.Methods: Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates.Results: A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation.Conclusion: Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature.
650 7a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
650 7a NATURVETENSKAPx Biologix Ekologi0 (SwePub)106112 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Ecology0 (SwePub)106112 hsv//eng
653 a Mosquito
653 a Malaria
653 a Microbiota
653 a Vector-borne disease
653 a Amazon forest
7001 a Marinotti, Osvaldou MTEKPrime, Aliso Viejo, CA USA.4 aut
7001 a Serrao, Deidre Machadou Univ Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil.4 aut
7001 a Correa, Laura Vianau Univ Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil.4 aut
7001 a Katak, Ricardo de Melou Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut
7001 a Juan, Campos-de-Oliveiru Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut
7001 a Muniz, Veranilce Alvesu Univ Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil.4 aut
7001 a de Oliveira, Marta Rodrigues4 aut
7001 a Ferreira, Joaquimu INPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil.4 aut
7001 a Pessoa, Marcos Cezar Fernandesu Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut
7001 a Roque, Rosemary Aparecidau INPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil.4 aut
7001 a da Mota, Adolfo Joseu Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut
7001 a Onorati, Pierou Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för ekologi,Department of Ecology,Swedish Univ Agr Sci SLU, Dept Ecol, Box 7044, S-75007 Uppsala, Sweden.4 aut
7001 a Souza-Neto, Jayme A.u Sao Paulo State Univ UNESP, Cent Multi User Lab, Dept Bioproc & Biotechnol, Sch Agr Sci, Botucatu, SP, Brazil.4 aut
7001 a Terenius, Olleu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Mikrobiologi,Swedish Univ Agr Sci SLU, Dept Ecol, Box 7044, S-75007 Uppsala, Sweden.,Institutionen för ekologi,Department of Ecology,Uppsala University4 aut0 (Swepub:slu)47992
7001 a Tadei, Wanderli Pedrou INPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil.4 aut
7102 a Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.b MTEKPrime, Aliso Viejo, CA USA.4 org
7102 a Sveriges lantbruksuniversitet
7730 t Malaria Journald : BioMed Central (BMC)g 20q 20x 1475-2875
8564 u https://doi.org/10.1186/s12936-020-03574-1y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1531419/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://malariajournal.biomedcentral.com/track/pdf/10.1186/s12936-020-03574-1
8564 u https://pub.epsilon.slu.se/id/eprint/23270/contentsx primaryx Raw objectx freey FULLTEXT
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-435198
8564 8u https://doi.org/10.1186/s12936-020-03574-1
8564 8u https://res.slu.se/id/publ/109965

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