SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Söderberg Nauclér Cecilia)
 

Sökning: WFRF:(Söderberg Nauclér Cecilia) > Active cytomegalovi...

  • Gredmark-Russ, SaraKarolinska Institutet (författare)

Active cytomegalovirus infection in aortic smooth muscle cells from patients with abdominal aortic aneurysm

  • Artikel/kapitelEngelska2009

Förlag, utgivningsår, omfång ...

  • 2008-12-16
  • Springer Science and Business Media LLC,2009
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-110127
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-110127URI
  • https://doi.org/10.1007/s00109-008-0413-4DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:118583738URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Cytomegalovirus (CMV) is associated with atherosclerosis and transplant vascular sclerosis. The aim of this study was to explore the hypothesis that active CMV infection in the vessel wall could be associated with abdominal aortic aneurysm (AAA). We examined the prevalence of CMV in AAA specimens from 22 patients undergoing surgery and, in five cases, characterized the function of smooth muscle cells (SMCs) from the aneurysm in vitro. Twenty-one (95%) of the 22 AAA specimens were CMV positive by a polymerase chain reaction assay, in situ hybridization, or a highly sensitive immunohistochemical staining technique. No positive cells were found in aortas from three CMV-seronegative organ donor cadavers. CMV immediate-early and late antigens were expressed in SMCs in the lesions and were associated with 5-lipoxygenase (5-LO) expression. CMV-positive intimal SMCs migrated 6.6 +/- 1.5 times more efficiently than CMV-negative medial SMCs (p < 0.05). In vitro CMV infection of medial SMCs resulted in a 3.2 +/- 1.2 times increase in migration (p < 0.05). The intimal migration was significantly inhibited by antibodies against basic fibroblast growth factor (bFGF; p < 0.05) in a dose-dependent fashion. Antibodies against platelet-derived growth factor (PDGF)-AB, insulin-like growth factor 1, vascular endothelial growth factor (VEGF), RANTES, monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein (MIP)-1alpha, or interleukin-1beta did not significantly affect intimal SMC migration. However, intimal and medial SMCs secreted similar amounts of bFGF, MCP-1, MIP-1alpha, RANTES, PDGF-AB, PDGF-BB, epidermal growth factor, and VEGF. CMV infection in vitro of intimal and medial cells did not result in significant changes of bFGF or MCP-1 secretion. Since CMV infection can affect several functional parameters in SMCs, including several key factors in infected SMCs, our findings provide support for the hypothesis that CMV contributes to the pathogenesis of abdominal aortic aneurysm.

Ämnesord och genrebeteckningar

  • MEDICINE
  • MEDICIN

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Dzabic, MensurKarolinska Institutet (författare)
  • Rahbar, AfsarKarolinska Institutet (författare)
  • Wanhainen, AndersUppsala universitet,Kärlkirurgi(Swepub:uu)anwan103 (författare)
  • Björck, MartinUppsala universitet,Kärlkirurgi(Swepub:uu)mabjo425 (författare)
  • Larsson, ErikUppsala universitet,Institutionen för immunologi, genetik och patologi(Swepub:uu)eriklars (författare)
  • Michel, Jean-Baptiste (författare)
  • Söderberg-Nauclér, CeciliaKarolinska Institutet (författare)
  • Karolinska InstitutetKärlkirurgi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal of Molecular Medicine: Springer Science and Business Media LLC87:4, s. 347-3560946-27161432-1440

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy