WFRF:(Scharfmann R)
Search: WFRF:(Scharfmann R) > Electrophysiologica...
- 4 of 5
- Previous record
- Next record
- To hitlist
Electrophysiological properties of human beta-cell lines EndoC-beta H1 and -beta H2 conform with human beta-cells
- Hastoy, B. (author)
- Godazgar, M. (author)
- Clark, A. (author)
- show more...
- Nylander, V. (author)
- Spiliotis, I. (author)
- van de Bunt, M. (author)
- Chibalina, M. V. (author)
- Barrett, A. (author)
- Burrows, C. (author)
- Tarasov, A. I. (author)
- Scharfmann, R. (author)
- Gloyn, A. L. (author)
- show less...
- (creator_code:org_t)
- 2018-11-19
- 2018
- English.
- In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 8
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Limited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-beta H1 and EndoC-beta H2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters that constitute the glucose-triggering pathway of insulin release. Both cell lines respond to glucose (6 and 20 mM) with 2- to 3-fold stimulation of insulin secretion which correlated with an elevation of [Ca2+](i), membrane depolarisation and increased action potential firing. Similar to human primary beta cells, K-ATP channel activity is low at 1mM glucose and is further reduced upon increasing glucose concentration; an effect that was mimicked by the K-ATP channel blocker tolbutamide. The upstroke of the action potentials reflects the activation of Ca2+ channels with some small contribution of TTX-sensitive Na+ channels. The repolarisation involves activation of voltage-gated Kv2.2 channels and large-conductance Ca2+-activated K+ channels. Exocytosis presented a similar kinetics to human primary beta cells. The ultrastructure of these cells shows insulin vesicles composed of an electrondense core surrounded by a thin clear halo. We conclude that the EndoC-beta H1 and -beta H2 cells share many features of primary human beta-cells and thus represent a useful experimental model.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- insulin-secretion
- glucose
- responses
- Science & Technology - Other Topics
- an pm
- 1973
- diabetologia
- v9
- p115
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Scientific Reports (Search for host publication in LIBRIS)
To the university's database
- 4 of 5
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Hastoy, B.
- Godazgar, M.
- Clark, A.
- Nylander, V.
- Spiliotis, I.
- van de Bunt, M.
- show more...
- Chibalina, M. V.
- Barrett, A.
- Burrows, C.
- Tarasov, A. I.
- Scharfmann, R.
- Gloyn, A. L.
- Rorsman, Patrik, ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Physiology
- Articles in the publication
- Scientific Repor ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
