Non-linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose

Brackmann, Christian,1973Chalmers tekniska högskola,Chalmers University of Technology (författare)

Non-linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non-linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering.

Dahlberg, Jan-Olof,1985Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)janolofd (författare)
Vrana, N. E.Dublin City University (författare)
Lally, C.Dublin City University (författare)
Gatenholm, Paul,1956Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)gatenh (författare)
Enejder, Annika,1969Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)enejder (författare)
Chalmers tekniska högskolaDublin City University (creator_code:org_t)

Av författaren/redakt...: Brackmann, Chris ...; Dahlberg, Jan-Ol ...; Vrana, N. E.; Lally, C.; Gatenholm, Paul, ...; Enejder, Annika, ...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.