Search: L773:1477 2000 OR L773:1478 0933 >
Rat alveolar and in...
Rat alveolar and interstitial macrophages in the fibrosing stage following quartz exposure
-
Zetterberg, G (author)
-
- Elmberger, G (author)
- Karolinska Institutet
-
Johansson, A (author)
-
show more...
-
- Lundahl, J (author)
- Karolinska Institutet
-
Lundborg, M (author)
-
- Skold, CM (author)
- Karolinska Institutet
-
- Tornling, G (author)
- Karolinska Institutet
-
Camner, P (author)
-
- Eklund, A (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- 2016-07-02
- 2000
- English.
-
In: Human & experimental toxicology. - : SAGE Publications. - 0960-3271 .- 1477-0903. ; 19:7, s. 402-411
- Related links:
-
http://kipublication...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Exposure to quartz induces pulmonary inflammation and development of fibrosis. In order to study the fibrosing process, we investigated morphology function and phenotype of alveolar (AMs) and interstitial (IMs) macrophages at an early stage of fibrosis in rats. Rats were exposed by intratracheal instillations of 10 mg quartz (n = 8) or saline (n = 8) and studied 3 months later. AMs were obtained by bronchoalveolar lavage and IMs by mechanical fragmentation, followed by enzymatic digestion of lung tissue. Histology revealed subacute silicosis, with early focal fibrosis and alveolar lipoproteinosis. AM quartz exposure increased phagocytic activity and expression of major histocompatibility complex (MHC) Ia antigens, the latter being associated with cellular antigen presenting capacity. IM had an even more pronounced expression of MHC than AM after quartz exposure. Both macrophage fractions had a higher expression of OX-42 (complement receptor 3, CR3) than controls, but the increase in the IM fraction might be explained by the remaining AM in the IM fraction. Exposed AM adhered less to extracellular matrix components (vitronectin and fibronectin) than controls. In contrast, the adhesion of IM to vitronectin increased after exposure. Besides increased adhesion, the effects on IM were scarce. Our results therefore do not support the hypothesis that IM has a key role in the process of inflammation, including fibrosis.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database