Purification and characterisation of NAD+-dependent xylitol dehydrogenase from Fusarium oxysporum

Panagiotou, G (författare): Department of Chemical Engineering, National Technical University of Athens

Kekos, D (författare): Department of Chemical Engineering, National Technical University of Athens

Macris, B.J (författare): Department of Chemical Engineering, National Technical University of Athens

Christakopoulos, Paul (författare)

(creator_code:org_t)

2002
2002
Engelska.
Ingår i: Biotechnology letters. - 0141-5492 .- 1573-6776. ; 24:24, s. 2089-2092

Relaterad länk:: https://urn.kb.se/re...; visa fler...; https://doi.org/10.1...; visa färre...

Abstract Ämnesord

Stäng

An NAD+-dependent xylitol dehydrogenase (XDH) from Fusarium oxysporum, a key enzyme in the conversion of xylose to ethanol, was purified to homogeneity and characterised. It was homodimeric with a subunit of Mr 48 000, and pI 3.6. It was optimally active at 45°C and pH 9-10. It was fully stable at pH 6-7 for 24 h and 30°C. Km values for D-xylitol and NAD+ were 94 mM and 0.14 mM, respectively. Mn2+ at 10 mM increased XDH activity 2-fold and Cu2+ at 10 mM inhibited activity completely.

Av författaren/redakt...: Panagiotou, G; Kekos, D; Macris, B.J; Christakopoulos, ...

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