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Sökning: WFRF:(Nilsson Magnus 1975 ) > Reduction of the HI...

  • Lindkvist, AnnicaKarolinska Institutet (författare)

Reduction of the HIV-1 reservoir in resting CD4+ T-lymphocytes by high dosage intravenous immunoglobulin treatment: a proof-of-concept study

  • Artikel/kapitelEngelska2009

Förlag, utgivningsår, omfång ...

  • 2009-07-01
  • Springer Science and Business Media LLC,2009
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:gup.ub.gu.se/112074
  • https://gup.ub.gu.se/publication/112074URI
  • https://doi.org/10.1186/1742-6405-6-15DOI
  • https://research.chalmers.se/publication/112074URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:219570221URI

Kompletterande språkuppgifter

  • Språk:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • BACKGROUND: The latency of HIV-1 in resting CD4+ T-lymphocytes constitutes a major obstacle for the eradication of virus in patients on antiretroviral therapy (ART). As yet, no approach to reduce this viral reservoir has proven effective. METHODS: Nine subjects on effective ART were included in the study and treated with high dosage intravenous immunoglobulin (IVIG) for five consecutive days. Seven of those had detectable levels of replication-competent virus in the latent reservoir and were thus possible to evaluate. Highly purified resting memory CD4+ T-cells were activated and cells containing replication-competent HIV-1 were quantified. HIV-1 from plasma and activated memory CD4+ T-cells were compared with single genome sequencing (SGS) of the gag region. T-lymphocyte activation markers and serum interleukins were measured. RESULTS: The latent HIV-1 pool decreased with in median 68% after IVIG was added to effective ART. The reservoir decreased in five, whereas no decrease was found in two subjects with detectable virus. Plasma HIV-1 RNA >or= 2 copies/mL was detected in five of seven subjects at baseline, but in only one at follow-up after 8-12 weeks. The decrease of the latent HIV-1 pool and the residual plasma viremia was preceded by a transitory low-level increase in plasma HIV-1 RNA and serum interleukin 7 (IL-7) levels, and followed by an expansion of T regulatory cells. The magnitude of the viral increase in plasma correlated to the size of the latent HIV-1 pool and SGS of the gag region showed that viral clones from plasma clustered together with virus from activated memory T-cells, pointing to the latent reservoir as the source of HIV-1 RNA in plasma. CONCLUSION: The findings from this uncontrolled proof-of-concept study suggest that the reservoir became accessible by IVIG treatment through activation of HIV-1 gene expression in latently-infected resting CD4+ T-cells. We propose that IVIG should be further evaluated as an adjuvant to effective ART.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Edén, Arvid,1975Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg(Swepub:gu)xedear (författare)
  • Norström, Melissa MKarolinska Institutet (författare)
  • Gonzalez, Veronica DKarolinska Institutet (författare)
  • Nilsson, Staffan,1956Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper, matematisk statistik,Department of Mathematical Sciences, Mathematical Statistics,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)staffan (författare)
  • Svennerholm, Bo,1949Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg(Swepub:gu)xsvebo (författare)
  • Karlsson, Annika CKarolinska Institutet (författare)
  • Sandberg, Johan KKarolinska Institutet (författare)
  • Sönnerborg, AndersKarolinska Institutet (författare)
  • Gisslén, Magnus,1962Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg(Swepub:gu)xgissm (författare)
  • Karolinska InstitutetInstitutionen för biomedicin, avdelningen för infektionssjukdomar (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:AIDS research and therapy: Springer Science and Business Media LLC6:11742-6405

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