SwePub
Sök i LIBRIS databas

  Utökad sökning

L773:1530 437X
 

Sökning: L773:1530 437X > Flex Printed Circui...

  • Damiati, SamarKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab,King Abdulaziz Univ, Dept Biochem, Fac Sci, Jeddah 21589, Saudi Arabia.;KTH Royal Inst Technol, Dept Prot Sci, Sci Life Lab, Div Nanobiotechnol, S-17121 Stockholm, Sweden. (författare)

Flex Printed Circuit Board Implemented Grapene-Based DNA Sensor for Detection of SARS-CoV-2

  • Artikel/kapitelEngelska2021

Förlag, utgivningsår, omfång ...

  • Institute of Electrical and Electronics Engineers (IEEE),2021
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-298758
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-298758URI
  • https://doi.org/10.1109/JSEN.2021.3068922DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20221019
  • Since the COVID-19 outbreak was declared a pandemic by the World Health Organization (WHO) in March 2020, ongoing efforts have been made to develop sensitive diagnostic platforms. Detection of viral RNA provides the highest sensitivity and specificity for detection of early and asymptomatic infections. Thus, this work aimed at developing a label-free genosensor composed of graphene as a working electrode that could be embedded into a flex printed circuit board (FPCB) for the rapid, sensitive, amplification-free and label-free detection of SARS-CoV-2. To facilitate liquid handling and ease of use, the developed biosensor was embedded with a user-friendly reservoir chamber. As a proof-of-concept, detection of a synthetic DNA strand matching the sequence of ORF1ab was performed as a two-step strategy involving the immobilization of a biotinylated complementary sequence on a streptavidin-modified surface, followed by hybridization with the target sequence recorded by the differential pulse voltammetric (DPV) technique in the presence of a ferro/ferricyanide redox couple. The effective design of the sensing platform improved its selectivity and sensitivity and allowed DNA quantification ranging from 100 fg/mL to 1 mu g/mL. Combining the electrochemical technique with FPCB enabled rapid detection of the target sequence using a small volume of the sample (5-20 mu L). We achieved a limit-of-detection of 100 fg/mL, whereas the predicted value was similar to 33 fg/mL, equivalent to approximately 5 x 10(5) copies/mL and comparable to sensitivities provided by isothermal nucleic acid amplification tests. We believe that the developed approach proves the ability of an FPCB-implemented DNA sensor to act as a potentially simpler and more affordable diagnostic assay for viral infections in Point-Of-Care (POC) applications.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Sopstad, SindreUniv South Eastern Norway, Fac Technol Nat Sci & Maritime, Dept Microsyst, N-3184 Borre, Norway. (författare)
  • Peacock, MartinZimmer & Peacock Ltd, Royston SG8 9JL, England. (författare)
  • Akhtar, Ahmad SaleemKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1pgxhk8 (författare)
  • Pinto, Ines FernandesKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1g105sd (författare)
  • Soares, Ruben R. G.KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u166jk6w (författare)
  • Russom, Aman,Prof.1976-KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1go6jxm (författare)
  • KTHNanobioteknologi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:IEEE Sensors Journal: Institute of Electrical and Electronics Engineers (IEEE)21:12, s. 13060-130671530-437X1558-1748

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy