Effect of nitric oxide on histamine-induced cytological transformations in parietal cells in isolated human gastric glands

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Fältnamn	Indikatorer	Metadata
000		03220naa a2200325 4500
001		oai:DiVA.org:liu-40472
003		SwePub
008		091010s2007 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-404722 URI
024	7	a https://doi.org/10.1007/s10620-006-9439-z2 DOI
040		a (SwePub)liu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Berg, Anna,d 1975-u Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)annbe52
245	1 0	a Effect of nitric oxide on histamine-induced cytological transformations in parietal cells in isolated human gastric glands
264		c 2006-12-14
264	1	b Springer Science and Business Media LLC,c 2007
338		a print2 rdacarrier
520		a Previous studies have shown that nitric oxide (NO) inhibits histamine-induced gastric acid secretion in isolated human gastric glands. NO synthase has been found to be present in the human oxyntic mucosa and has been suggested to serve as a paracrine regulator of gastric acid secretion. Histamine stimulation of parietal cells induces cytoskeletal rearrangements, recruitment of H +/K +-ATPase-rich tubulovesicles to the apical membrane and expansion of intracellular canaliculi. The aim of the present study was thus to investigate (i) the effect of an NO donor on histamine-induced cytological transformations and (ii) the influence of increased [Ca 2+] i on NO-induced morphological changes in human parietal cells. Human gastric glands were isolated and subjected to the NO donor SNAP prior to histamine administration. [Ca 2+] i was increased by photolysis of the caged Ca 2+ compound NP-EGTA. The distribution of F-actin, ezrin, and H +/K +-ATPase was assessed by confocal microscopy. Ultrastructural analysis was performed using transmission electron microscopy. SNAP did not influence the histamine-induced translocation of F-actin, ezrin, and H +/K +-ATPase but prevented an increase in the canalicular size. Elevation of [Ca 2+] i in resting cells was found to mimic histamine-induced intraparietal cell transformations; however, NO-induced parietal cell morphology was unaffected by a rise in [Ca 2+] i. These results indicate that NO inhibits secretion of fluid into the canalicular lumen without affecting membrane recruitment and that this effect is Ca 2+-insensitive. © 2006 Springer Science+Business Media, Inc.
653		a MEDICINE
653		a MEDICIN
700	1	a Redéen, Stefan,d 1961-u Linköpings universitet,Kirurgi,Hälsouniversitetet4 aut0 (Swepub:liu)stere32
700	1	a Sjöstrand, Sven-Erik,d 1938-u Linköpings universitet,Farmakologi,Hälsouniversitetet4 aut0 (Swepub:liu)svesj65
700	1	a Ericson, Ann-Charlott,d 1965-u Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)anner00
710	2	a Linköpings universitetb Cellbiologi4 org
773	0	t Digestive Diseases and Sciencesd : Springer Science and Business Media LLCg 52:1, s. 126-136q 52:1<126-136x 0163-2116x 1573-2568
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-40472
856	4 8	u https://doi.org/10.1007/s10620-006-9439-z

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