Flow cytometric analysis of BHRF1 expression prohibiting apoptosis induced by radiation

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Fältnamn	Indikatorer	Metadata
000		01975naa a2200313 4500
001		oai:prod.swepub.kib.ki.se:1942746
003		SwePub
008		240811s1999 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:19427462 URI
024	7	a https://doi.org/10.1177/0003489499108005112 DOI
040		a (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Huang, H4 aut
245	1 0	a Flow cytometric analysis of BHRF1 expression prohibiting apoptosis induced by radiation
264		c 2016-06-29
264	1	b SAGE Publications,c 1999
520		a The Epstein-Barr virus gene BHRF1 has homology with proto-oncogene bcl-2, which can protect cells from apoptosis. In order to investigate the effect of BHRF1 expression on the anti-apoptotic ability of nasopharyngeal carcinoma (NPC) cells after irradiation, a high—BHRF1 expression vector was constructed and transfected into the NPC cell line CNE2. Then, the alteration of proliferation and apoptosis in the cells was tested by flow cytometry after cobalt 60 irradiation. The results showed that BHRF1 expression could increase G1 delay and decrease the cell percentage in S phase before irradiation, and reduce the apoptotic rate of CNE2 cells and increase the cell percentage in S phase after irradiation. The results suggest that BHRF1 expression is able to alter the cell cycle and protect CNE2 cells from apoptosis induced by radiation.
700	1	a Pan, XH4 aut
700	1	a Zhou, SM4 aut
700	1	a Li, ZJ4 aut
700	1	a Yu, L4 aut
700	1	a Kong, XT4 aut
700	1	a Zheng, QP4 aut
773	0	t The Annals of otology, rhinology, and laryngologyd : SAGE Publicationsg 108:5, s. 481-484q 108:5<481-484x 0003-4894x 1943-572X
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:1942746
856	4 8	u https://doi.org/10.1177/000348949910800511

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Av författaren/redakt...: Huang, H; Pan, XH; Zhou, SM; Li, ZJ; Yu, L; Kong, XT; visa fler...; Zheng, QP; visa färre...

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