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Case Studies Exempl...
Case Studies Exemplifying the Transition to Animal Component-free Cell Culture
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- Weber, Tilo (författare)
- German Animal Welfare Federation
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- Wiest, Joachim (författare)
- Cellasys GmbH
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- Oredsson, Stina (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Zoofysiologi,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Animal Physiology,Lund University Research Groups
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- Bieback, Karen (författare)
- Heidelberg University
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(creator_code:org_t)
- 2022-08-19
- 2022
- Engelska 9 s.
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Ingår i: Alternatives to Laboratory Animals. - : SAGE Publications. - 0261-1929 .- 2632-3559. ; 50:5, s. 330-338
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cell culture techniques are strongly connected with modern scientific laboratories and production facilities. Thus, choosing the most suitable medium for the cells involved is vital, not only directly to optimise cell viability but also indirectly to maximise the reliability of the experiments performed with the cells. Fetal bovine or calf serum (FBS or FCS, respectively) is the most commonly used cell culture medium supplement, providing various nutritional factors and macromolecules essential for cell growth. Yet, the use of FBS encompasses a number of disadvantages. Scientifically, one of the most severe disadvantages is the lot-to-lot variability of animal sera that hampers reproducibility. Therefore, transitioning from the use of these ill-defined, component-variable, inconsistent, xenogenic, ethically questionable and even potentially infectious media supplements, is key to achieving better data reproducibility and thus better science. To demonstrate that the transition to animal component-free cell culture is possible and achievable, we highlight three different scenarios and provide some case studies of each, namely: i) the adaptation of single cell lines to animal component-free culture conditions by the replacement of FBS and trypsin; ii) the adaptation of multicellular models to FBS-free conditions; and (iii) the replacement of FBS with human platelet lysate (hPL) for the generation of primary stem/stromal cell cultures for clinical purposes. By highlighting these examples, we aim to foster and support the global movement towards more consistent science and provide evidence that it is indeed possible to step out of the currently smouldering scientific reproducibility crisis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Nyckelord
- animal component-free
- cell cultures
- chemically defined medium
- FBS-free
- fetal bovine serum
- human platelet lysate
- multicellular models
- reproducibility crisis
- transition
- xeno-free
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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