Reduced Gene Expression Levels of Munc13-1 and Additional Components of the Presynaptic Exocytosis Machinery Upon Conditional Targeting of Vglut2 in the Adolescent Mouse

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Fältnamn	Indikatorer	Metadata
000		03843naa a2200433 4500
001		oai:DiVA.org:uu-237282
003		SwePub
008		141201s2014 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2372822 URI
024	7	a https://doi.org/10.1002/syn.217762 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Rajagopalan, Aparnau Uppsala universitet,Genetisk utvecklingsbiologi4 aut
245	1 0	a Reduced Gene Expression Levels of Munc13-1 and Additional Components of the Presynaptic Exocytosis Machinery Upon Conditional Targeting of Vglut2 in the Adolescent Mouse
264		c 2014-09-26
264	1	b Wiley,c 2014
338		a print2 rdacarrier
520		a Presynaptic proteins orchestrate an intricate interplay of dynamic interactions in order to regulate quantal exocytosis of transmitter-filled vesicles, and their dysregulation might cause neurological and neuropsychiatric dysfunction. Mice carrying a spatiotemporal restriction in the expression of the Vesicular glutamate transporter 2 (Vglut2; aka Slc17a6) in the cortex, amygdala and hippocampal subiculum from the third postnatal week show a strong anxiolytic phenotype and certain behavioral correlates of schizophrenia. To further understand the molecular consequences of this targeted deletion of Vglut2, we performed an unbiased microarray analysis comparing gene expression levels in the subiculum of these conditional Vglut2 knockout mice (Vglut2(f/f;CamKII) cKO) to those in control littermates. Expression of Unc13C (Munc13-3), a member of the Unc/Munc family, previously shown to be important for glutamatergic transmission, was identified to be significantly down-regulated. Subsequent analysis by quantitative RT-PCR revealed a 50% down-regulation of Munc 13-1, the gene encoding the Unc/Munc subtype described as an essential component in the majority of glutamtergic synapses in the hippocampus. Genes encoding additional components of the presynaptic machinery were also found regulated, including Rab3A, RIM1, as well as Syntaxin1 and Synaptobrevin. Altered expression levels of these genes were further found in the amygdala and in the retrosplenial group of the cortex, additional regions in which Vglut2 was conditionally targeted. These findings suggest that expression levels of Vglut2 might be important for the maintenance of gene expression in the presynaptic machinery in the adult mouse brain. Synapse 68:624-633, 2014.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng
653		a amygdala
653		a cortex
653		a hippocampus
653		a microarray
653		a schizophrenia
653		a Slc17a6
653		a subiculum
700	1	a Schweizer, Nadineu Uppsala universitet,Genetisk utvecklingsbiologi4 aut0 (Swepub:uu)nadsc454
700	1	a Nordenankar, Karinu Uppsala universitet,Genetisk utvecklingsbiologi,Funktionell farmakologi4 aut0 (Swepub:uu)karno631
700	1	a Jahan, Sultana Nilufaru Uppsala universitet,Genetisk utvecklingsbiologi4 aut
700	1	a Emilsson, Linau Uppsala universitet,Genetisk utvecklingsbiologi,Evolution och utvecklingsbiologi4 aut0 (Swepub:uu)linaemil
700	1	a Wallen-Mackenzie, Åsau Uppsala universitet,Genetisk utvecklingsbiologi4 aut0 (Swepub:uu)asmac168
710	2	a Uppsala universitetb Genetisk utvecklingsbiologi4 org
773	0	t Synapsed : Wileyg 68:12, s. 624-633q 68:12<624-633x 0887-4476x 1098-2396
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-237282
856	4 8	u https://doi.org/10.1002/syn.21776

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