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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004656naa a2200577 4500
001oai:DiVA.org:uu-138967
003SwePub
008101221s2011 | |||||||||||000 ||eng|
009oai:slubar.slu.se:58944
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1389672 URI
0247 a https://doi.org/10.1002/jsfa.43432 DOI
0247 a https://res.slu.se/id/publ/589442 URI
040 a (SwePub)uud (SwePub)slu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Wessman, Peru Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Department of Mircobiology, SLU, Swedish University of Agricultural Sciences4 aut0 (Swepub:slu)47496
2451 0a Impact of matrix properties on survival of freeze-dried bacteria
264 c 2011-03-28
264 1b Wiley,c 2011
338 a print2 rdacarrier
520 a Background: Disaccharides are in general first choice as formulation compounds when freezedrying microorganisms. Although polysaccharides and other biopolymers are considered too large to stabilize and interact with cell components in the same beneficial way as disaccharides, polymers have been reported to support cell survival. In the present study we compare the efficiency of sucrose, the polymers Ficoll, hydroxyethylcellulose, hydroxypropylmethylcellulose and polyvinylalcohol to support survival of three bacterial strains during freeze-drying. The initial osmotic conditions were adjusted to be similar for all formulations. Formulation characterization was used to interpret the impact that different compound properties had on cell survival. Results: Despite differences in molecular size, both sucrose and the sucrose based polymer Ficoll supported cell survival after freeze-drying equally well. All formulations became amorphous upon dehydration. Scanning electron microscopy and X-ray diffraction data showed that the discerned differences in structure of the dry formulations had little impact on the survival rates. The capability of the polymers to support cell survival correlated with the surface activity of the polymers in a similar way for all investigated bacterial strains. Conclusion: Polymer-based formulations can support cell survival as effectively as disaccharides if formulation properties of importance for maintaining cell viability are identified and controlled.
650 7a LANTBRUKSVETENSKAPERx Lantbruksvetenskap, skogsbruk och fiskex Livsmedelsvetenskap0 (SwePub)401032 hsv//swe
650 7a AGRICULTURAL SCIENCESx Agriculture, Forestry and Fisheriesx Food Science0 (SwePub)401032 hsv//eng
653 a formulation
653 a freeze-drying
653 a biopolymer
653 a sucrose
653 a bacteria
653 a surface
653 a Food science
653 a Livsmedelsvetenskap
653 a TECHNOLOGY
653 a TEKNIKVETENSKAP
653 a Biologi med inriktning mot mikrobiologi
653 a Biology with specialization in Microbiology
653 a Teknisk fysik med inriktning mot materialvetenskap
653 a Engineering Science with specialization in Materials Science
7001 a Mahlin, Dennyu Uppsala universitet,Institutionen för farmaci4 aut0 (Swepub:uu)dma10394
7001 a Akhtar, Sultanu Uppsala universitet,Tillämpad materialvetenskap4 aut0 (Swepub:uu)sulak695
7001 a Rubino, Stefano,d 1978-u Uppsala universitet,Tillämpad materialvetenskap4 aut0 (Swepub:uu)steru726
7001 a Leifer, Klaus,d 1965-u Uppsala universitet,Tillämpad materialvetenskap4 aut0 (Swepub:uu)kllei839
7001 a Kessler, Vadimu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kemi,Department of Chemistry,Department of Chemistry, SLU, Swedish University of Agricultural Sciences4 aut0 (Swepub:slu)46900
7001 a Håkansson, Sebastianu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Department of Microbiology, SLU, Swedish University of Agricultural Sciences4 aut0 (Swepub:slu)48672
7102 a Sveriges lantbruksuniversitetb Institutionen för mikrobiologi4 org
7102 a Sveriges lantbruksuniversitet
7730 t Journal of the Science of Food and Agricultured : Wileyg 91:14, s. 2518-2528q 91:14<2518-2528x 0022-5142x 1097-0010
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-138967
8564 8u https://doi.org/10.1002/jsfa.4343
8564 8u https://res.slu.se/id/publ/58944

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