Sökning: WFRF:(Nister Monica) > Molecular Genetic a...
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000 | 03381naa a2200481 4500 | |
001 | oai:DiVA.org:uu-123034 | |
003 | SwePub | |
008 | 100422s2010 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:120214644 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1230342 URI |
024 | 7 | a https://doi.org/10.1111/j.1365-2990.2010.01070.x2 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1202146442 URI |
040 | a (SwePub)uud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Qu, Mingqiu Uppsala universitet,Neurologi4 aut |
245 | 1 0 | a Molecular Genetic and Epigenetic analysis of NCX2/SLC8A2 at 19q13.3 in Human Gliomas |
264 | 1 | b Wiley,c 2010 |
338 | a print2 rdacarrier | |
520 | a AimLoss of heterozygosity (LOH) at 19q13.3 is a common genetic change in human gliomas, indicating yet unknown glial-specific tumour suppressor genes in this chromosome region. NCX2/SLC8A2 located on chromosome 19q13.32 encodes a Na(+)/Ca(2+)exchanger, which contributes to intracellular Ca(2+)homeostasis. Its expression is restricted to brain, and it is neither present in other normal tissues nor in gliomas at any significant level. The aim of this study was to investigate if NCX2 might be a tumour suppressor gene involved in glioma.MethodsWe performed a systematic analysis of NCX2 in 42 human gliomas using microsatellite analysis for evaluation of LOH at 19q, DNA sequencing and DNA methylation analysis.ResultsExcept for three known intragenic SNPs, rs12459087, rs7259674, and rs8104926, no NCX2 sequence variations were detected in any of the tumour samples. Furthermore, a CpG island in the 5' promoter region of NCX2 was unmethylated. Interestingly, the CpG sites of three gene-body CpG islands located in exon 2, intron 2-3 and exon 3 and of a 5' CpG rich area relevant to so called CpG island shore of NCX2 were methylated in all 8 glioma samples and in 3 established glioma cell lines tested. Surprisingly, NCX2 could be activated by addition of the DNA methylation inhibitor 5-aza-2'-deoxycytidine to glioma cell lines in which NCX2 was completely silent.ConclusionResults indicate that DNA methylation may play a key role in the transcriptional silencing of NCX2. | |
653 | a 19q13.3 | |
653 | a DNA methylation | |
653 | a Glioma | |
653 | a Mutation | |
653 | a NCX2 | |
653 | a Single nucleotide polymorphism | |
653 | a MEDICINE | |
653 | a MEDICIN | |
653 | a Neurology | |
653 | a Neurologi | |
700 | 1 | a Jiao, Hongu Karolinska Institutet4 aut |
700 | 1 | a Zhao, Jianu Karolinska Institutet4 aut |
700 | 1 | a Ren, Zhi-Pingu Uppsala universitet,Institutionen för genetik och patologi4 aut |
700 | 1 | a Smits, Anjau Uppsala universitet,Neurologi4 aut0 (Swepub:uu)anjasmit |
700 | 1 | a Kere, Juhau Karolinska Institutet4 aut |
700 | 1 | a Nistér, Monicau Karolinska Institutet4 aut |
710 | 2 | a Uppsala universitetb Neurologi4 org |
773 | 0 | t Neuropathology and Applied Neurobiologyd : Wileyg 36:3, s. 198-210q 36:3<198-210x 0305-1846x 1365-2990 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-123034 |
856 | 4 8 | u https://doi.org/10.1111/j.1365-2990.2010.01070.x |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:120214644 |
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