Acrylamide alters CREB and retinoic acid signaling pathways during differentiation of the human neuroblastoma SH-SY5Y cell line

Attoff, Kristina, 1985- (author): Stockholms universitet,Institutionen för biokemi och biofysik

Östlund Farrants, Ann-Kristin (author): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

Johansson, Ylva (author): Stockholms universitet,Institutionen för biokemi och biofysik

Cediel Ulloa, Andrea (author)

Lundqvist, Jessica (author): Stockholms universitet,Institutionen för biokemi och biofysik

Forsby, Anna (author): Stockholms universitet,Institutionen för biokemi och biofysik

(creator_code:org_t)

Abstract Subject headings

Acrylamide is a known neurotoxic compound that we get exposed to through food and through the environment. It can cross the placental barrier as well as the blood-brain barrier resulting in exposure of the fetus and the infant child. We used the human neuroblastoma cell line SH-SY5Y to study the effects of non-cytotoxic acrylamide exposure during 9 days of differentiation on two differentially important signaling pathways, i.e. the retinoic acid receptor (RAR) and cAMP response element-binding protein (CREB) signaling in neurons. Our results showed that exposure of non-cytotoxic concentrations of acrylamide during 9 days of differentiation induced altered expression of multiple genes that are part of the CREB and RAR activation pathways, e.g. cellular retinoic acid binding protein 1, retinol binding protein 7, CREB5 and fibroblast growth factor receptor 2. Other well-established neuronal markers such as brain-derived neurotrophic factor, syntaxin binding protein 2, transforming growth factor beta 1, the dopaminergic markers monoamine oxidase A and dopamine receptor D2 as wells as the cholinergic marker choline O-acetyltransferase were also significantly altered by acrylamide. Our results reveal that acrylamide interferes with crucial pathways involved in neuronal differentiation in vitro and raise concerns over the potential toxic outcomes in humans.

By the author/editor: Attoff, Kristina ...; Östlund Farrants ...; Johansson, Ylva; Cediel Ulloa, An ...; Lundqvist, Jessi ...; Forsby, Anna

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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