Sökning: WFRF:(Hedman Johannes) >
Evaluation of the V...
Evaluation of the VISAGE basic tool for appearance and ancestry inference using ForenSeq® chemistry on the MiSeq FGx® system
-
- Xavier, Catarina (författare)
- Medical University of Innsbruck
-
- de la Puente, Maria (författare)
- Medical University of Innsbruck,University of Santiago de Compostela
-
- Sidstedt, Maja (författare)
- Swedish Police Authority
-
visa fler...
-
- Junker, Klara (författare)
- Swedish Police Authority
-
- Minawi, Angelika (författare)
- Federal Criminal Police Office, Wiesbaden
-
- Unterländer, Martina (författare)
- Federal Criminal Police Office, Wiesbaden
-
- Chantrel, Yann (författare)
- Institut National de Police Scientifique
-
- Laurent, François Xavier (författare)
- Institut National de Police Scientifique
-
- Delest, Anna (författare)
- Institut National de Police Scientifique
-
- Hohoff, Carsten (författare)
- Institut für Forensische Genetik GmbH (IFG)
-
- Bastisch, Ingo (författare)
- Federal Criminal Police Office, Wiesbaden
-
- Hedman, Johannes (författare)
- Lund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Swedish Police Authority
-
- van der Gaag, Kristiaan J. (författare)
- Netherlands Forensic Institute
-
- Sijen, Titia (författare)
- Netherlands Forensic Institute,University of Amsterdam
-
- Parson, Walther (författare)
- Pennsylvania State University,Medical University of Innsbruck
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- Engelska.
-
Ingår i: Forensic Science International: Genetics. - : Elsevier BV. - 1872-4973. ; 58
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
http://www.fsigeneti...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The possibility of providing investigative leads when conventional DNA identification methods fail to solve a case can be of extreme relevance to law enforcement. Therefore, the forensic genetics community has focused research towards the broadened use of DNA, particularly for prediction of appearance traits, bio-geographical ancestry and age. The VISible Attributes through GEnomics (VISAGE) Consortium expanded the use of DNA phenotyping by developing new molecular and statistical tools for appearance, age and ancestry prediction. The VISAGE basic tool for appearance (EVC) and ancestry (BGA) prediction was initially developed using Ampliseq chemistry, but here is being evaluated using ForenSeq chemistry. The VISAGE basic tool offers a total of 41 EVC and 115 BGA SNPs and thus provides more predictions, i.e., skin color, than achieved with the ForenSeq DNA Signature Prep kit that is based on 24 EVC and 56 BGA SNPs. Five VISAGE laboratories participated in collaborative experiments to provide foreground for developmental validation of the assay. Assessment of assay performance and quality metrics, reproducibility, sensitivity, inhibitor tolerance and species specificity are described. Furthermore, the assay was tested using challenging samples such as mock casework samples and artificially degraded DNA. Two different analysis strategies were applied for this study and output on genotype calls and read depth was compared. Overall, inter-laboratory, inter-method and concordance with publicly available data were analysed and compared. Finally, the results showed a reliable and robust tool, which can be easily applied for laboratories already using a MiSeq FGx with ForenSeq reagents.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Annan medicin och hälsovetenskap -- Rättsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Other Medical and Health Sciences -- Forensic Science (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Xavier, Catarina
-
de la Puente, Ma ...
-
Sidstedt, Maja
-
Junker, Klara
-
Minawi, Angelika
-
Unterländer, Mar ...
-
visa fler...
-
Chantrel, Yann
-
Laurent, Françoi ...
-
Delest, Anna
-
Hohoff, Carsten
-
Bastisch, Ingo
-
Hedman, Johannes
-
van der Gaag, Kr ...
-
Sijen, Titia
-
Parson, Walther
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Annan medicin oc ...
-
och Rättsmedicin
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Medicinsk geneti ...
- Artiklar i publikationen
-
Forensic Science ...
- Av lärosätet
-
Lunds universitet