DNA-lipid systems. An amphiphile self-assembly and polymer-surfactant perspective

Lindman, BjörnLund University,Lunds universitet,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH (författare)

A1501
The interaction between DNA and oppositely charged surfactants has been investigated by several techniques, like fluorescence microscopy, electron microscopy, phase diagram determination, and ellipsometry. The phase behaviour is more strongly associative than that in previously studied systems. A precipitate is formed for very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of positively charged vesicles and that the addition of anionic surfactant can release free DNA back into solution from a compact globular complex between DNA and cationic surfactant. Finally, we investigated DNA interactions with polycations, chitosans with different chain lengths, by fluorescence microscopy, in vivo transfections assays and cryogenic transmission electron microscopy. The general conclusion is that a chitosan effective in promoting compaction is also efficient in transfection.

Mel'nikov, S (författare)
Mel'nikova, Y (författare)
Nylander, TommyLund University,Lunds universitet,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)fk1-tny (författare)
Eskilsson, KRISE,YKI – Ytkemiska institutet (författare)
Miguel, M (författare)
Dias, R (författare)
Leal, C (författare)
Fysikalisk kemiEnheten för fysikalisk och teoretisk kemi (creator_code:org_t)

Ingår i:Lipid and Polymer-Lipid SystemsBerlin, Heidelberg : Springer Berlin Heidelberg120, s. 52-630340-255X1437-80279783540430018

Av författaren/redakt...: Lindman, Björn; Mel'nikov, S; Mel'nikova, Y; Nylander, Tommy; Eskilsson, K; Miguel, M; visa fler...; Dias, R; Leal, C; visa färre...

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