Monitoring of Protein Biomarkers of Inflammation in Human Traumatic Brain Injury Using Microdialysis and Proximity Extension Assay Technology in Neurointensive Care

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: L773:1557 9042 OR L773:0897 7151 > Monitoring of Prote...

Details
MARC

Dyhrfort, PhilipUppsala universitet,Enblad: Neurokirurgi (author)

Monitoring of Protein Biomarkers of Inflammation in Human Traumatic Brain Injury Using Microdialysis and Proximity Extension Assay Technology in Neurointensive Care

Article/chapterEnglish2019

Publisher, publication year, extent ...

MARY ANN LIEBERT, INC,2019
electronicrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:uu-396069
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-396069URI
https://doi.org/10.1089/neu.2018.6320DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Traumatic brain injury (TBI) is followed by secondary injury mechanisms strongly involving neuroinflammation. To monitor the complex inflammatory cascade in human TBI, we used cerebral microdialysis (MD) and multiplex proximity extension assay (PEA) technology and simultaneously measured levels of 92 protein biomarkers of inflammation in MD samples every three hours for five days in 10 patients with severe TBI under neurointensive care. One mu L MD samples were incubated with paired oligonucleotide-conjugated antibodies binding to each protein, allowing quantification by real-time quantitative polymerase chain reaction. Sixty-nine proteins were suitable for statistical analysis. We found five different patterns with either early (<48 h; e.g., CCL20, IL6, LIF, CCL3), mid (48-96 h; e.g., CCL19, CXCL5, CXCL10, MMP1), late (>96 h; e.g., CD40, MCP2, MCP3), biphasic peaks (e.g., CXCL1, CXCL5, IL8) or stable (e.g., CCL4, DNER, VEGFA)/low trends. High protein levels were observed for e.g., CXCL1, CXCL10, MCP1, MCP2, IL8, while e.g., CCL28 and MCP4 were detected at low levels. Several proteins (CCL8, -19, -20, -23, CXCL1, -5, -6, -9, -11, CST5, DNER, Flt3L, and SIRT2) have not been studied previously in human TBI. Cross-correlation analysis revealed that LIF and CXCL5 may play a central role in the inflammatory cascade. This study provides a unique data set with individual temporal trends for potential inflammatory biomarkers in patients with TBI. We conclude that the combination of MD and PEA is a powerful tool to map the complex inflammatory cascade in the injured human brain. The technique offers new possibilities of protein profiling of complex secondary injury pathways.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Neurologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Neurology hsv//eng
biomarkers
inflammation
microdialysis
molecular tools
neurointensive care
proteomics
traumatic brain injury

Added entries (persons, corporate bodies, meetings, titles ...)

Shen, QiujinUppsala universitet,Molekylära verktyg(Swepub:uu)qiush740 (author)
Clausen, Fredrik,1973-Uppsala universitet,Institutionen för neurovetenskap,Uppsala Univ, Dept Neurosci, Sect Neurosurg, Uppsala, Sweden,Enblad:neurokirurgi(Swepub:uu)fredclau (author)
Thulin, Måns,1986-Uppsala universitet,Statistiska institutionen,Univ Edinburgh, Sch Math, Edinburgh, Midlothian, Scotland;Univ Edinburgh, Maxwell Inst Math Sci, Edinburgh, Midlothian, Scotland(Swepub:uu)maner971 (author)
Enblad, PerUppsala universitet,Enblad: Neurokirurgi(Swepub:uu)perenbla (author)
Kamali-Moghaddam, MasoodUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi(Swepub:uu)masookam (author)
Lewén, Anders,1965-Uppsala universitet,Enblad: Neurokirurgi(Swepub:uu)andelewe (author)
Hillered, Lars,1952-Uppsala universitet,Institutionen för neurovetenskap(Swepub:uu)larshill (author)
Uppsala universitetEnblad: Neurokirurgi (creator_code:org_t)

Related titles

In:Journal of Neurotrauma: MARY ANN LIEBERT, INC36:20, s. 2872-28850897-71511557-9042

Internet link

Find in a library

Journal of Neurotrauma (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Dyhrfort, Philip; Shen, Qiujin; Clausen, Fredrik ...; Thulin, Måns, 19 ...; Enblad, Per; Kamali-Moghaddam ...; show more...; Lewén, Anders, 1 ...; Hillered, Lars, ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Neurology

Articles in the publication: Journal of Neuro ...

By the university: Uppsala University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se