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Sökning: L773:1557 9042 OR L773:0897 7151 > Monitoring of Prote...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004533naa a2200481 4500
001oai:DiVA.org:uu-396069
003SwePub
008191030s2019 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3960692 URI
0247 a https://doi.org/10.1089/neu.2018.63202 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Dyhrfort, Philipu Uppsala universitet,Enblad: Neurokirurgi4 aut0 (Swepub:uu)phidy397
2451 0a Monitoring of Protein Biomarkers of Inflammation in Human Traumatic Brain Injury Using Microdialysis and Proximity Extension Assay Technology in Neurointensive Care
264 1b MARY ANN LIEBERT, INC,c 2019
338 a electronic2 rdacarrier
520 a Traumatic brain injury (TBI) is followed by secondary injury mechanisms strongly involving neuroinflammation. To monitor the complex inflammatory cascade in human TBI, we used cerebral microdialysis (MD) and multiplex proximity extension assay (PEA) technology and simultaneously measured levels of 92 protein biomarkers of inflammation in MD samples every three hours for five days in 10 patients with severe TBI under neurointensive care. One mu L MD samples were incubated with paired oligonucleotide-conjugated antibodies binding to each protein, allowing quantification by real-time quantitative polymerase chain reaction. Sixty-nine proteins were suitable for statistical analysis. We found five different patterns with either early (<48 h; e.g., CCL20, IL6, LIF, CCL3), mid (48-96 h; e.g., CCL19, CXCL5, CXCL10, MMP1), late (>96 h; e.g., CD40, MCP2, MCP3), biphasic peaks (e.g., CXCL1, CXCL5, IL8) or stable (e.g., CCL4, DNER, VEGFA)/low trends. High protein levels were observed for e.g., CXCL1, CXCL10, MCP1, MCP2, IL8, while e.g., CCL28 and MCP4 were detected at low levels. Several proteins (CCL8, -19, -20, -23, CXCL1, -5, -6, -9, -11, CST5, DNER, Flt3L, and SIRT2) have not been studied previously in human TBI. Cross-correlation analysis revealed that LIF and CXCL5 may play a central role in the inflammatory cascade. This study provides a unique data set with individual temporal trends for potential inflammatory biomarkers in patients with TBI. We conclude that the combination of MD and PEA is a powerful tool to map the complex inflammatory cascade in the injured human brain. The technique offers new possibilities of protein profiling of complex secondary injury pathways.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Neurologi0 (SwePub)302072 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Neurology0 (SwePub)302072 hsv//eng
653 a biomarkers
653 a inflammation
653 a microdialysis
653 a molecular tools
653 a neurointensive care
653 a proteomics
653 a traumatic brain injury
7001 a Shen, Qiujinu Uppsala universitet,Molekylära verktyg4 aut0 (Swepub:uu)qiush740
7001 a Clausen, Fredrik,d 1973-u Uppsala universitet,Institutionen för neurovetenskap,Uppsala Univ, Dept Neurosci, Sect Neurosurg, Uppsala, Sweden,Enblad:neurokirurgi4 aut0 (Swepub:uu)fredclau
7001 a Thulin, Måns,d 1986-u Uppsala universitet,Statistiska institutionen,Univ Edinburgh, Sch Math, Edinburgh, Midlothian, Scotland;Univ Edinburgh, Maxwell Inst Math Sci, Edinburgh, Midlothian, Scotland4 aut0 (Swepub:uu)maner971
7001 a Enblad, Peru Uppsala universitet,Enblad: Neurokirurgi4 aut0 (Swepub:uu)perenbla
7001 a Kamali-Moghaddam, Masoodu Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)masookam
7001 a Lewén, Anders,d 1965-u Uppsala universitet,Enblad: Neurokirurgi4 aut0 (Swepub:uu)andelewe
7001 a Hillered, Lars,d 1952-u Uppsala universitet,Institutionen för neurovetenskap4 aut0 (Swepub:uu)larshill
7102 a Uppsala universitetb Enblad: Neurokirurgi4 org
7730 t Journal of Neurotraumad : MARY ANN LIEBERT, INCg 36:20, s. 2872-2885q 36:20<2872-2885x 0897-7151x 1557-9042
8564 u https://doi.org/10.1089/neu.2018.6320y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1366697/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://www.liebertpub.com/doi/pdf/10.1089/neu.2018.6320
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-396069
8564 8u https://doi.org/10.1089/neu.2018.6320

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