Sökning: WFRF:(Arnqvist Hans 1943 ) > Inhibition of purom...
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000 | 03409naa a2200289 4500 | |
001 | oai:DiVA.org:liu-23811 | |
003 | SwePub | |
008 | 091007s2004 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-238112 URI |
040 | a (SwePub)liu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Söderlund, Gustav4 aut |
245 | 1 0 | a Inhibition of puromycin-induced apoptosis in breast cancer cells by IGF-I occurs simultaneously with increased protein synthesis |
264 | 1 | c 2004 |
338 | a print2 rdacarrier | |
520 | a The objective of the following work was to study the apoptosis inducing effect of puromycin in MCF-7 breast cancer cells and compare this effect with cycloheximide and emetine, 2 other inhibitors of protein synthesis. We also wished to investigate if the apoptosis modulating effect of insulin-like growth factor-1 (IGF-I) was similar for the 3 inhibitors. An immunological assay, quantifying mono- and oligonucleosome fragments and morphological criteria after nuclear staining, were used to study apoptosis. Protein synthesis was measured by incorporation of 3H-leucine in the cells, and solution hybridization and Western blot were performed to estimate IGF-I receptor m-RNA and IGF-I receptor protein respectively. Puromycin at 0.5 μg/ml induced a high level of apoptosis in MCF-7 breast cancer cells, although there was still a non-negligible amount of synthesized protein. In the case of cycloheximide and emetine, apoptosis occured when protein synthesis was almost completely blocked. IGF-I at a concentration of 10 ng/ml significantly reduced the level of apoptosis induced by puromycin, emetine, or cycloheximide. We also noticed a parallel increase in 3H-leucine incorporation when apoptosis induced by puromycin was lowered as an effect of IGF-I, in contrast to cycloheximide and emetine where IGF-I reduced the apoptosis level without increasing the 3H-leucine incorporation. At a higher concentration of puromycin (5. 7 μg/ml), which blocked protein synthesis, IGF-I at 10 ng/ml did not reduce apoptosis. The level of IGF-I receptor m-RNA was not influenced by the use of a concentration of puromycin (0.5 μg/ml) inducing a high degree of apoptosis. These results suggest, that reduction of puromycin-induced apoptosis by IGF-I occurs simultaneously with increased protein synthesis, in contrast to emetine and cycloheximide. Furthermore it would appear that puromycin-induced apoptosis is not caused by reduced levels of IGF-I receptors. | |
653 | a MEDICINE | |
653 | a MEDICIN | |
700 | 1 | a Haarhaus, Mathias,d 1967-u Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Njurmedicin,Njurmedicinska kliniken US4 aut0 (Swepub:liu)matha60 |
700 | 1 | a Chisalita, Ioana Simona,d 1972-u Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi4 aut0 (Swepub:liu)ioach17 |
700 | 1 | a Arnqvist, Hans,d 1943-u Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi,Endokrin- och magtarmmedicinska kliniken US4 aut0 (Swepub:liu)hanar64 |
710 | 2 | a Linköpings universitetb Hälsouniversitetet4 org |
773 | 0 | t Neoplasma (Bratislava)g 51:1q 51:1x 0028-2685x 1338-4317 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-23811 |
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