Inhibition of puromycin-induced apoptosis in breast cancer cells by IGF-I occurs simultaneously with increased protein synthesis

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Fältnamn	Indikatorer	Metadata
000		03409naa a2200289 4500
001		oai:DiVA.org:liu-23811
003		SwePub
008		091007s2004 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-238112 URI
040		a (SwePub)liu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Söderlund, Gustav4 aut
245	1 0	a Inhibition of puromycin-induced apoptosis in breast cancer cells by IGF-I occurs simultaneously with increased protein synthesis
264	1	c 2004
338		a print2 rdacarrier
520		a The objective of the following work was to study the apoptosis inducing effect of puromycin in MCF-7 breast cancer cells and compare this effect with cycloheximide and emetine, 2 other inhibitors of protein synthesis. We also wished to investigate if the apoptosis modulating effect of insulin-like growth factor-1 (IGF-I) was similar for the 3 inhibitors. An immunological assay, quantifying mono- and oligonucleosome fragments and morphological criteria after nuclear staining, were used to study apoptosis. Protein synthesis was measured by incorporation of 3H-leucine in the cells, and solution hybridization and Western blot were performed to estimate IGF-I receptor m-RNA and IGF-I receptor protein respectively. Puromycin at 0.5 μg/ml induced a high level of apoptosis in MCF-7 breast cancer cells, although there was still a non-negligible amount of synthesized protein. In the case of cycloheximide and emetine, apoptosis occured when protein synthesis was almost completely blocked. IGF-I at a concentration of 10 ng/ml significantly reduced the level of apoptosis induced by puromycin, emetine, or cycloheximide. We also noticed a parallel increase in 3H-leucine incorporation when apoptosis induced by puromycin was lowered as an effect of IGF-I, in contrast to cycloheximide and emetine where IGF-I reduced the apoptosis level without increasing the 3H-leucine incorporation. At a higher concentration of puromycin (5. 7 μg/ml), which blocked protein synthesis, IGF-I at 10 ng/ml did not reduce apoptosis. The level of IGF-I receptor m-RNA was not influenced by the use of a concentration of puromycin (0.5 μg/ml) inducing a high degree of apoptosis. These results suggest, that reduction of puromycin-induced apoptosis by IGF-I occurs simultaneously with increased protein synthesis, in contrast to emetine and cycloheximide. Furthermore it would appear that puromycin-induced apoptosis is not caused by reduced levels of IGF-I receptors.
653		a MEDICINE
653		a MEDICIN
700	1	a Haarhaus, Mathias,d 1967-u Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Njurmedicin,Njurmedicinska kliniken US4 aut0 (Swepub:liu)matha60
700	1	a Chisalita, Ioana Simona,d 1972-u Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi4 aut0 (Swepub:liu)ioach17
700	1	a Arnqvist, Hans,d 1943-u Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi,Endokrin- och magtarmmedicinska kliniken US4 aut0 (Swepub:liu)hanar64
710	2	a Linköpings universitetb Hälsouniversitetet4 org
773	0	t Neoplasma (Bratislava)g 51:1q 51:1x 0028-2685x 1338-4317
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-23811

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