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Pressure-Related Ef...
Pressure-Related Effects on Homeostatic Müller Cell Proteins in the Adult Porcine in Vitro Retina
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- Svare, Frida (author)
- Lund University,Lunds universitet,Oftalmologi (Malmö),Forskargrupper vid Lunds universitet,Oftalmologi, Ghosh,Ophthalmology (Malmö),Lund University Research Groups,Oftalmology, Ghosh
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- Ghosh, Fredrik (author)
- Lund University,Lunds universitet,Oftalmologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Oftalmologi, Ghosh,Forskargrupper vid Lunds universitet,Ophthalmology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Oftalmology, Ghosh,Lund University Research Groups
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(creator_code:org_t)
- 2024
- 2024
- English 11 s.
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In: Current Eye Research. - 0271-3683. ; 49:3, s. 303-313
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Abstract
Subject headings
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- Purpose: To explore early pressure-related effects on Müller cell homeostatic proteins in the in vitro adult porcine retina. Methods: Retinal explants were subjected to 0-, 10-, 30-, or 60-mmHg of pressure for 24 or 48 h in culture. Retinal explants fixed immediately after enucleation were used as controls. Müller cell proteins were evaluated by GFAP, GS, CRALBP, and bFGF immunohistochemistry. Results: GFAP-labeling revealed no differences in fluorescence intensity after 24 or 48 h in any of the pressure groups compared with control retinas. However, a higher intensity was found in the 30- and 60-mmHg groups compared with 0-mmHg counterparts after 24 and 48 h. A higher intensity in GS-labeled sections was found in the 10-and 60-mmHg groups compared with controls and remaining pressure groups after 48 h. Compared with control retinas, CRALBP labeling revealed a higher intensity in the 60-mmHg group after 24 h and in the 10-, 30-, and 60-mmHg groups after 48 h. After 24 and 48 h, a lower intensity was found in bFGF-labeled cells in the 0-, 10-, and 30-mmHg groups compared with controls, while no difference was seen for the 60-mmHg group. Conclusions: Müller cells in the cultured porcine adult retina respond early to pressure by altering the expression of GFAP as well as the homeostatic proteins GS, CRALBP, and bFGF.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
Keyword
- GFAP
- Müller cells
- Pressure-related effects
- retinal explants
Publication and Content Type
- art (subject category)
- ref (subject category)
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