Feature-extraction from endopeptidase cleavage sites in mitochondrial targeting peptides

• Cleavage sites in nuclear-encoded mitochondrial protein targeting peptides (mTPs) from mammals, yeast, and plants have been analysed for characteristic physicochemical features using statistical methods, perceptrons, multilayer neural networks, and self-organizing feature maps, Three different sequence motifs were found, revealing loosely defined arginine motifs with Arg in positions -10, -3, and -2. A self-organizing feature map was able to cluster these three types of endopeptidase target sites but did not identify any species-specific characteristics in mTPs, Neural networks were used to define local sequence features around precursor cleavage sites.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Keyword

kohonen network

mitochondrial processing peptidase (MPP)

mitochondrial intermediate peptidase (MIP)

neural network

protein import

sequence motif

mitochondrial targeting

Publication and Content Type

ref (subject category)

art (subject category)