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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004056naa a2200529 4500
001oai:research.chalmers.se:a080d5c1-65b9-41da-aa87-ad294bc6d381
003SwePub
008180920s2018 | |||||||||||000 ||eng|
0247 a https://research.chalmers.se/publication/5052262 URI
0247 a https://research.chalmers.se/publication/5050322 URI
0247 a https://doi.org/10.1016/j.cell.2018.07.0132 DOI
040 a (SwePub)cth
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Yu, Tao,d 1986u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)taoy
2451 0a Reprogramming Yeast Metabolism from Alcoholic Fermentation to Lipogenesis
264 1b Elsevier BV,c 2018
338 a electronic2 rdacarrier
520 a Engineering microorganisms for production of fuels and chemicals often requires major re-programming of metabolism to ensure high flux toward the product of interest. This is challenging, as millions of years of evolution have resulted in establishment of tight regulation of metabolism for optimal growth in the organism's natural habitat. Here, we show through metabolic engineering that it is possible to alter the metabolism of Saccharomyces cerevisiae from traditional ethanol fermentation to a pure lipogenesis metabolism, resulting in high-level production of free fatty acids. Through metabolic engineering and process design, we altered subcellular metabolic trafficking, fine tuned NADPH and ATP supply, and decreased carbon flux to biomass, enabling production of 33.4 g/L extracellular free fatty acids. We further demonstrate that lipogenesis metabolism can replace ethanol fermentation by deletion of pyruvate decarboxylase enzymes followed by adaptive laboratory evolution. Genome sequencing of evolved strains showed that pyruvate kinase mutations were essential for this phenotype.
650 7a TEKNIK OCH TEKNOLOGIERx Kemiteknikx Kemiska processer0 (SwePub)204012 hsv//swe
650 7a ENGINEERING AND TECHNOLOGYx Chemical Engineeringx Chemical Process Engineering0 (SwePub)204012 hsv//eng
650 7a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
650 7a TEKNIK OCH TEKNOLOGIERx Industriell bioteknikx Biokatalys och enzymteknik0 (SwePub)209062 hsv//swe
650 7a ENGINEERING AND TECHNOLOGYx Industrial Biotechnologyx Biocatalysis and Enzyme Technology0 (SwePub)209062 hsv//eng
653 a adaptive laboratory evolution
653 a fatty acids
653 a synthetic biology
653 a metabolism
653 a metabolic engineering
653 a Crabtree effect
653 a lipid
7001 a Zhou, Yongjin,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)yongjin
7001 a Huang, Mingtao,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)mingtao
7001 a Liu, Quanli,d 1988u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)quanli
7001 a Pereira, Rui,d 1986u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)ruip
7001 a David, Florian,d 1981u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)davidfl
7001 a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nielsenj
7102 a Chalmers tekniska högskola4 org
7730 t Celld : Elsevier BVg 174:6, s. 1549-1572q 174:6<1549-1572x 0092-8674x 1097-4172
8564 u https://research.chalmers.se/publication/505226/file/505226_Fulltext.pdfx primaryx freey FULLTEXT
8564 u http://www.cell.com/article/S0092867418309073/pdf
8564 8u https://research.chalmers.se/publication/505226
8564 8u https://research.chalmers.se/publication/505032
8564 8u https://doi.org/10.1016/j.cell.2018.07.013

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