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Sökning: WFRF:(Karlsson Erik A.) > (2010-2014) > Activation of NF-ka...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004505naa a2200433 4500
001oai:DiVA.org:oru-32903
003SwePub
008140102s2012 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-329032 URI
0247 a https://doi.org/10.1074/jbc.M112.3862842 DOI
040 a (SwePub)oru
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Pradhan, Ajay,d 1983-u Örebro universitet,Institutionen för naturvetenskap och teknik4 aut0 (Swepub:oru)aypn
2451 0a Activation of NF-kappa B Protein Prevents the Transition from Juvenile Ovary to Testis and Promotes Ovarian Development in Zebrafish
264 1b The American Society for Biochemistry and Molecular Biology,c 2012
338 a print2 rdacarrier
500 a Funding Agency: Orebro University; Agri-Food and Veterinary Authority; Temasek Life Sciences Laboratory, Singapore (se även Forskningsfinansiärer)
520 a Testis differentiation in zebrafish involves juvenile ovary to testis transformation initiated by an apoptotic wave. The molecular regulation of this transformation process is not fully understood. NF-kappa B is activated at an early stage of development and has been shown to interact with steroidogenic factor-1 in mammals, leading to the suppression of anti-Mullerian hormone (Amh) gene expression. Because steroidogenic factor-1 and Amh are important for proper testis development, NF-kappa B-mediated induction of anti-apoptotic genes could, therefore, also play a role in zebrafish gonad differentiation. The aim of this study was to examine the potential role of NF-kappa B in zebrafish gonad differentiation. Exposure of juvenile zebrafish to heat-killed Escherichia coli activated the NF-kappa B pathways and resulted in an increased ratio of females from 30 to 85%. Microarray and quantitative real-time-PCR analysis of gonads showed elevated expression of NF-kappa B-regulated genes. To confirm the involvement of NF-kappa B-induced anti-apoptotic effects, zebrafish were treated with sodium deoxycholate, a known inducer of NF-kappa B or NF-kappa B activation inhibitor (NAI). Sodium deoxycholate treatment mimicked the effect of heat-killed bacteria and resulted in an increased proportion of females from 25 to 45%, whereas the inhibition of NF-kappa B using NAI resulted in a decrease in females from 45 to 20%. This study provides proof for an essential role of NF-kappa B in gonadal differentiation of zebrafish and represents an important step toward the complete understanding of the complicated process of sex differentiation in this species and possibly other cyprinid teleosts as well.
650 7a NATURVETENSKAPx Biologix Utvecklingsbiologi0 (SwePub)106142 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Developmental Biology0 (SwePub)106142 hsv//eng
653 a Biology
653 a Biologi
7001 a Khalaf, Hazem,d 1981-u Örebro universitet,Institutionen för hälsovetenskap och medicin4 aut0 (Swepub:oru)hkf
7001 a Ochsner, Scott A.u Baylor College of Medicine, Houston, USA4 aut
7001 a Sreenivasan, Rajiniu Temasek Life Sciences Laboratory, Singapore, Singapore4 aut
7001 a Koskinen, Jarno,d 1973-u Örebro universitet,Institutionen för naturvetenskap och teknik4 aut0 (Swepub:oru)jokn
7001 a Karlsson, Marie,d 1977-u Örebro universitet,Institutionen för naturvetenskap och teknik4 aut0 (Swepub:oru)mekn
7001 a Karlsson, Jesperu Department of Biology, School of Science and Technology, Örebro University, Örebro, Sweden4 aut
7001 a McKenna, Neil J.u Baylor College of Medicine, Houston, USA4 aut
7001 a Orban, Laszlou Temasek Life Sciences Laboratory, Singapore, Singapore; National University of Singapore, Singapore, Singapore; University of Pannonia, Keszthely, Hungary4 aut
7001 a Olsson, Per-Erik,d 1957-u Örebro universitet,Institutionen för naturvetenskap och teknik4 aut0 (Swepub:oru)pkon
7102 a Örebro universitetb Institutionen för naturvetenskap och teknik4 org
7730 t Journal of Biological Chemistryd : The American Society for Biochemistry and Molecular Biologyg 287:45, s. 37926-37938q 287:45<37926-37938x 0021-9258x 1083-351X
8564 u https://doi.org/10.1074/jbc.M112.386284y Fulltext
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-32903
8564 8u https://doi.org/10.1074/jbc.M112.386284

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