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A combination of th...
A combination of the activation marker CD86 and the immune checkpoint marker B and T lymphocyte attenuator (BTLA) indicates a putative permissive activation state of B cell subtypes in healthy blood donors independent of age and sex
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- Axelsson, Susanne (författare)
- Department of Pathology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden
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- Magnuson, Anders (författare)
- Clinical Epidemiology and Biostatistics, School of Medical Sciences, Örebro University, Örebro, Sweden
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- Lange, Anna, 1975- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Infectious Diseases
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- Alshamari, Aseel (författare)
- Department of Clinical Immunology and Transfusion Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden
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- Hultgren Hörnquist, Elisabeth, 1965- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper
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- Hultgren, Olof, 1970- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Clinical Immunology and Transfusion Medicine
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(creator_code:org_t)
- 2020-03-20
- 2020
- Engelska.
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Ingår i: BMC Immunology. - : BioMed Central (BMC). - 1471-2172. ; 21:1
- Relaterad länk:
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https://doi.org/10.1...
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https://bmcimmunol.b...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BACKGROUND: The use of anti-B cell based therapies in immune-mediated diseases targeting general B cell markers or molecules important for B cell function has increased the clinical needs of monitoring B cell subpopulations.RESULTS: We analyzed the expression profile of cell surface markers CD86 and B and T lymphocyte attenuator (BTLA) in B cell subtypes using flow cytometry, including naïve, transitional, switched memory, non-switched memory and double-negative memory B cells and plasmablasts, and investigated the dependence of age and sex in a healthy adult blood donor population. The switched memory B cell subtype displayed a divergent expression of the markers, with increased CD86 and decreased BTLA as compared to non-switched and double negative memory cells, as well as compared to naïve B cells. Plasmablasts expressed highly increased CD86 compared to all other subtypes and a decreased expression of BTLA compared to naïve cells, but still higher compared to the memory cell populations. Transitional B cells had CD86 and BTLA expression similar to the other naïve cells.CONCLUSIONS: We show divergent expression of CD86 and BTLA in memory cells and plasmablasts compared to naïve B cells independent of age and sex. Furthermore, a similarly divergent difference of expression pattern was seen between the memory cell subtypes, altogether indicating that the combination of CD86 and BTLA might be markers for a permissive activation state. We suggest the combination of CD86 and BTLA expression on B cell subtypes as a potentially important tool in monitoring the status of B cell subtypes before and after treatments influencing the B cell compartment.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- B cell
- B cell subtype
- BTLA
- CD86
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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