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Structure and Compo...
Structure and Composition of Native Membrane Derived Polymer-Supported Lipid Bilayers
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- Pace, Hudson, 1982 (författare)
- Chalmers University of Technology, Sweden,Chalmers tekniska högskola
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- Hannestad, Jonas, 1981 (författare)
- RISE,Yta, process och formulering,Chalmers University of Technology, Sweden,Chalmers tekniska högskola,RISE Research Institutes of Sweden
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- Armanious, Antonius, 1981 (författare)
- Chalmers University of Technology, Sweden,Chalmers tekniska högskola
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- Adamo, Marco (författare)
- Institute Laue-Langevin, France ; Imperial College London, UK,Imperial College of Science, Technology and Medicine,Institut Laue-Langevin
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- Agnarsson, Björn, 1977 (författare)
- Chalmers University of Technology, Sweden,Chalmers tekniska högskola
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- Gunnarsson, Anders, 1981 (författare)
- AstraZeneca, Sweden,AstraZeneca AB
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- Micciulla, Samantha (författare)
- Institute Laue-Langevin, France ; Max Planck Institute of Colloids and Interfaces, Germany,Institut Laue-Langevin,Max Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG)
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- Sjövall, Peter (författare)
- RISE,Yta, process och formulering,Chalmers University of Technology, Sweden,Chalmers tekniska högskola,RISE Research Institutes of Sweden
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- Gerelli, Yuri (författare)
- Institute Laue-Langevin, France,Institut Laue-Langevin
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- Höök, Fredrik, 1966 (författare)
- Chalmers University of Technology, Sweden,Chalmers tekniska högskola
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(creator_code:org_t)
- 2018-10-15
- 2018
- Engelska.
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 90:21, s. 13065-13072
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Over the last two decades, supported lipid bilayers (SLBs) have been extensively used as model systems to study cell membrane structure and function. While SLBs have been traditionally produced from simple lipid mixtures, there has been a recent surge in compositional complexity to better mimic cellular membranes and thereby bridge the gap between classic biophysical approaches and cell experiments. To this end, native cellular membrane derived SLBs (nSLBs) have emerged as a new category of SLBs. As a new type of biomimetic material, an analytical workflow must be designed to characterize its molecular composition and structure. Herein, we demonstrate how a combination of fluorescence microscopy, neutron reflectometry, and secondary ion mass spectrometry offers new insights on structure, composition, and quality of nSLB systems formed using so-called hybrid vesicles, which are a mixture of native membrane material and synthetic lipids. With this approach, we demonstrate that the nSLB formed a continuous structure with complete mixing of the synthetic and native membrane components and a molecular stoichiometry that essentially mirrors that of the hybrid vesicles. Furthermore, structural investigation of the nSLB revealed that PEGylated lipids do not significantly thicken the hydration layer between the bilayer and substrate when on silicon substrates; however, nSLBs do have more topology than their simpler, purely synthetic counterparts. Beyond new insights regarding the structure and composition of nSLB systems, this work also serves to guide future researchers in producing and characterizing nSLBs from their cellular membrane of choice.
Ämnesord
- NATURVETENSKAP -- Kemi -- Oorganisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Inorganic Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Nyckelord
- Biomimetics
- Cell membranes
- Cytology
- Fluorescence microscopy
- Lipid bilayers
- Lipids
- Mixtures
- Secondary ion mass spectrometry
- Substrates
- Cellular membranes
- Compositional complexity
- Continuous structures
- Molecular compositions
- Neutron reflectometry
- Silicon substrates
- Structural investigation
- Supported lipid bilayers
- Structure (composition)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Pace, Hudson, 19 ...
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Hannestad, Jonas ...
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Armanious, Anton ...
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Adamo, Marco
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Agnarsson, Björn ...
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Gunnarsson, Ande ...
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visa fler...
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Micciulla, Saman ...
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Sjövall, Peter
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Gerelli, Yuri
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Höök, Fredrik, 1 ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Oorganisk kemi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Fysikalisk kemi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Fysik
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och Annan fysik
- Artiklar i publikationen
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Analytical Chemi ...
- Av lärosätet
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RISE
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Chalmers tekniska högskola