WFRF:(Niss Frida)
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Monitoring of Chromatin Organization at the Nuclear Pore Complex, Inner Nuclear Membrane, and Nuclear Interior in Live Cells by Fluorescence Ratiometric Imaging of Chromatin (FRIC)
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- Niss, Frida (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Bergqvist, Cecilia, 1986- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Ström, Anna-Lena, 1975- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- (creator_code:org_t)
- 2022-04-13
- 2022
- English.
- In: The Nuclear Pore Complex. - New York : Humana Press Inc.. - 9781071623367 - 9781071623374 ; , s. 151-160
- Book chapter (peer-reviewed)
Abstract
Subject headings
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- The image analysis tool FRIC (Fluorescence Ratiometric Imaging of Chromatin) quantitatively monitors dynamic spatiotemporal distribution of euchromatin and total chromatin in live cells. A vector (pTandemH) assures stoichiometrically constant expression of the histone variants Histone 3.3 and Histone 2B, fused to EGFP and mCherry, respectively. Quantitative ratiometric (H3.3/H2B) imaging displayed a concentrated distribution of heterochromatin in the periphery of U2OS cell nuclei. As a proof of concept, peripheral heterochromatin responded to experimental manipulation of histone acetylation as well as expression of the mutant lamin A protein “progerin,” which causes Hutchinson-Gilford Progeria Syndrome. In summary FRIC is versatile, unbiased, robust, requires a minimum of experimental steps and is suitable for screening purposes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Chromatin
- Fluorescence ratiometric
- Live imaging
- Nuclear membrane
- Nuclear pore complex
- histone
- lamin A
- cell nucleus
- cell nucleus membrane
- fluorescence
- genetics
- HeLa cell line
- heterochromatin
- human
- metabolism
- nuclear pore
- HeLa Cells
- Histones
- Humans
- Lamin Type A
- Nuclear Envelope
Publication and Content Type
- ref (subject category)
- kap (subject category)
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