WFRF:(Raap M.)
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LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02552naa a2200397 4500 | |
| 001 | oai:DiVA.org:uu-12453 | |
| 003 | SwePub | |
| 008 | 071221s2007 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-124532 URI |
| 024 | 7 | a https://doi.org/10.1007/978-3-540-73040-82 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a kon2 swepub-publicationtype |
| 100 | 1 | a Allalou, Aminu Uppsala universitet,Centrum för bildanalys,Datoriserad bildanalys4 aut0 (Swepub:uu)amial914 |
| 245 | 1 0 | a Image Based Measurements of Single Cell mtDNA Mutation Load |
| 264 | 1 | a Berlin, Heidelberg :b Springer Berlin Heidelberg,c 2007 |
| 338 | a print2 rdacarrier | |
| 520 | a Cell cultures as well as cells in tissue always display a certain degree of variability, and measurements based on cell averages will miss important information contained in a heterogeneous population. This paper presents automated methods for image based measurements of mitochondiral DNA (mtDNA) mutations in individual cells. The mitochondria are present in the cell’s cytoplasm, and each cytoplasm has to be delineated. Three different methods for segmentation of cytoplasms are compared and it is shown that automated cytoplasmic delineation can be performed 30 times faster than manual delineation, with an accuracy as high as 87%. The final image based measurements of mitochondrial mutation load are also compared to, and show high agreement with, measurements made using biochemical techniques. | |
| 650 | 7 | a NATURVETENSKAPx Data- och informationsvetenskapx Datorseende och robotik0 (SwePub)102072 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Computer and Information Sciencesx Computer Vision and Robotics0 (SwePub)102072 hsv//eng |
| 653 | a single cell analysis | |
| 653 | a cytoplasm segmentation | |
| 653 | a mitochondrial DNA | |
| 653 | a image cytometry | |
| 653 | a Image analysis | |
| 653 | a Bildanalys | |
| 700 | 1 | a van de Rijke, Frans M.4 aut |
| 700 | 1 | a Jahangir Tafrechi, Roos4 aut |
| 700 | 1 | a Raap, Anton K.4 aut |
| 700 | 1 | a Wählby, Carolinau Uppsala universitet,Centrum för bildanalys4 aut0 (Swepub:uu)cli05194 |
| 710 | 2 | a Uppsala universitetb Centrum för bildanalys4 org |
| 773 | 0 | t Image Analysis, Proceedingsd Berlin, Heidelberg : Springer Berlin Heidelbergg , s. 631-640q <631-640z 9783540730392 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-12453 |
| 856 | 4 8 | u https://doi.org/10.1007/978-3-540-73040-8 |
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