Sökning: WFRF:(Toonen A.) > Multi-level charact...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03118naa a2200577 4500 | |
001 | oai:DiVA.org:kth-209602 | |
003 | SwePub | |
008 | 170621s2017 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2096022 URI |
024 | 7 | a https://doi.org/10.1371/journal.pone.01785332 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Nadadhur, A. G.4 aut |
245 | 1 0 | a Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells |
264 | c 2017-06-06 | |
264 | 1 | b Public Library of Science,c 2017 |
338 | a print2 rdacarrier | |
500 | a QC 20170621 | |
520 | a Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory- inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory- inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a Ganglionic Eminence | |
653 | a Astrocyte Coculture | |
653 | a Differentiation | |
653 | a Interneurons | |
653 | a Brain | |
653 | a Gaba | |
653 | a Specification | |
653 | a Maturation | |
653 | a Glutamate | |
653 | a Disease | |
700 | 1 | a Melero, J. E.4 aut |
700 | 1 | a Meijer, M.4 aut |
700 | 1 | a Schut, D.4 aut |
700 | 1 | a Jacobs, G.4 aut |
700 | 1 | a Wan Li, K.4 aut |
700 | 1 | a Hjorth, Johannesu KTH,Beräkningsvetenskap och beräkningsteknik (CST),Science for Life Laboratory, SciLifeLab,Vrije Universiteit, Netherlands4 aut0 (Swepub:kth)u1timwkw |
700 | 1 | a Meredith, R. M.4 aut |
700 | 1 | a Toonen, R. F.4 aut |
700 | 1 | a Van Kesteren, R. E.4 aut |
700 | 1 | a Smit, A. B.4 aut |
700 | 1 | a Verhage, M.4 aut |
700 | 1 | a Heine, V. M.4 aut |
710 | 2 | a KTHb Beräkningsvetenskap och beräkningsteknik (CST)4 org |
773 | 0 | t PLOS ONEd : Public Library of Scienceg 12:6q 12:6x 1932-6203 |
856 | 4 | u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0178533&type=printable |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-209602 |
856 | 4 8 | u https://doi.org/10.1371/journal.pone.0178533 |
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