Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier

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Fältnamn	Indikatorer	Metadata
000		04055naa a2200397 4500
001		oai:DiVA.org:oru-12952
003		SwePub
008		110103s2010 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-129522 URI
024	7	a https://doi.org/10.1152/ajpgi.00327.20092 DOI
040		a (SwePub)oru
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Karczewski, Jurgenu Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands4 aut
245	1 0	a Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier
264	1	b American Physiological Society,c 2010
338		a print2 rdacarrier
520		a Lactobacillus plantarum, a commensal bacterium of humans, has been proposed to enhance the intestinal barrier, which is compromised in a number of intestinal disorders. To study the effect of L. plantarum strain WCFS1 on human barrier function, healthy subjects were administered L. plantarum or placebo in the duodenum for 6 h by means of a feeding catheter. The scaffold protein zonula occludens (ZO)-1 and transmembrane protein occludin were found to be significantly increased in the vicinity of the tight-junction (TJ) structures, which form the paracellular seal between cells of the epithelium. In an in vitro model of the human epithelium, L. plantarum induced translocation of ZO-1 to the TJ region; however, the effects on occludin were minor compared with those seen in vivo. L. plantarum was shown to activate Toll-like receptor 2 (TLR2) signaling, and treatment of Caco-2 monolayers with the TLR2 agonist Pam3-Cys-SK4(PCSK) significantly increased fluorescent staining of occludin in the TJ. Pretreatment of Caco-2 monolayers with L. plantarum or PCSK significantly attenuated the effects of phorbol ester-induced dislocation of ZO-1 and occludin and the associated increase in epithelial permeability. Our results identifying commensal bacterial stimulation of TLR2 in the gut epithelium as a regulator of epithelial integrity have important implications for understanding probiotic mechanisms and the control of intestinal homeostasis.
653		a epithelium
653		a intestine
653		a MEDICINE
653		a MEDICIN
653		a Medicine
653		a Medicin
700	1	a Troost, Freddy J.u TI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands4 aut
700	1	a Konings, Ireneu Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands4 aut
700	1	a Dekker, Janu TI Food & Nutr, Wageningen, Netherlands4 aut
700	1	a Kleerebezem, Michielu TI Food & Nutr, Wageningen, Netherlands; NIZO Food Res, Ede, Netherlands; Microbiol Lab, Univ Wageningen, Wageningen, Netherlands4 aut
700	1	a Brummer, Robertu Örebro universitet,Hälsoakademin,TI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands4 aut0 (Swepub:oru)rnbr
700	1	a Wells, Jerry M.u Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands4 aut
710	2	a Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlandsb TI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands4 org
773	0	t American Journal of Physiology - Gastrointestinal and Liver Physiologyd : American Physiological Societyg 298:6, s. G851-G859q 298:6<G851-G859x 0193-1857x 1522-1547
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-12952
856	4 8	u https://doi.org/10.1152/ajpgi.00327.2009

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Av författaren/redakt...: Karczewski, Jurg ...; Troost, Freddy J ...; Konings, Irene; Dekker, Jan; Kleerebezem, Mic ...; Brummer, Robert; visa fler...; Wells, Jerry M.; visa färre...

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Av lärosätet: Örebro universitet

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