Mapping the thermoresponses of micralgae by integrating single-cell arrays on a programmable temperature stage

• We report on a miniaturized temperature regulation system for the precise control of thermal environments inside microfluidic devices. This system uses a combination of Joule heating and active cooling to achieve defined temperatures to an affixed microfluidic device, allowing for rapid and cost-effective temperature control in single-use devices. Spatiotemporal temperature profiles in a biologically relevant temperature range (20-40 °C) are programmed in a custom software. We illustrate the capabilities of the system with a study of the coral symbiont Symbiodinium at elevated temperatures. Through monitoring of single-cell photophysiology, we evaluate the response to different applied temperature profile and the strain-specific thermal sensitivity thresholds.

Nyckelord

thermal stress

Symbiodinium

temperature regulation

single-cell

Joule heating

Teknisk fysik med inriktning mot mikrosystemteknik

Engineering Science with specialization in Microsystems Technology

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