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Development of a hi...
Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
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- Grey, Carl (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Edebrink, Per (författare)
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Krook, Maria (författare)
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- Jacobsson, Sven P. (författare)
- Stockholms universitet,Institutionen för analytisk kemi
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- Engelska.
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Ingår i: Journal of chromatography. B. - : Elsevier BV. - 1570-0232 .- 1873-376X. ; 877:20-21, s. 1827-1832
- Relaterad länk:
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- HPAEC-PAD
- MALDI-TOF MS
- Oligosaccharides
- N-glycosylation
- Glycoprotein
- Monoclonal antibodies
- Analytical chemistry
- Analytisk kemi
- Monoclonal antibodies
- Glycoprotein
- N-glycosylation
- Oligosaccharides
- HPAEC-PAD
- MALDI-TOF MS
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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