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Biochemical characterization of the multi-enzyme system produced by Penicillium decumbens grown on rutin
- Mamma, Diomi (author)
- Kalogeris, Emmanuel (author)
- Hatzinikolaou, Dimitris G (author)
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- Lekanidou, Afroditi (author)
- Kekos, Dimitris (author)
- Macris, Basil J (author)
- Christakopoulos, Paul (author)
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- 2004
- 2004
- English.
- In: Food biotechnology. - 0890-5436 .- 1532-4249. ; 18:1, s. 1-18
- Journal article (peer-reviewed)
Abstract
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- Penicillium decumbens produced a set of enzymes, including a monoxygenase and two glycosidases, which degrade rutin, a nontoxic flavonoid glycoside, to water-soluble products. The monoxygenase (quercetinase) cleaves the heterocyclic ring in quercetin, the aglycone part of rutin. The glycosidases (alpha-L-rhamnosidase and beta-glucosidase) hydrolyze the bonds between quercetin and rutinose, and between glucose and rhamnose, the constituent monosaccharides of rutinose. Simultaneous production of the three enzymes was optimized following the examination of a number of culture conditions. Maximum enzyme activities were observed when the fungus was grown at 30 °C with an initial pH of 7.0, using 8.0 g/L rutin and 9.0 g/L di-ammonium hydrogen phosphate as carbon and nitrogen sources, respectively. The enzymes were purified to electrophoretic homogeneity by a series of consecutive chromatographic steps including anion and cation exchange as well as gel filtration. The purified quercetinase revealed an apparent tetrameric structure, with a reduced molecular mass of 45 kDa. alpha-L-Rhamnosidase showed an apparent molecular mass of 58 kDa and the purified beta-glucosidase was a tetramer exhibiting a reduced molecular mass of 120 kDa.
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- Luleå University of Technology
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