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HIVIS-DNA or HIVISo...
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Hinkula, JormaLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,Department of Microbiology Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden
(författare)
HIVIS-DNA or HIVISopt-DNA priming followed by CMDR vaccinia-based boosts induce both humoral and cellular murine immune responses to HIV.
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
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Elsevier,2017
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-141182
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-141182URI
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https://doi.org/10.1016/j.heliyon.2017.e00339DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:228721397URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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BACKGROUND: In order to develop a more effective prophylactic HIV-1 vaccine it is important optimize the components, improve Envelope glycoprotein immunogenicity as well as to explore prime-boost immunization schedules. It is also valuable to include several HIV-1 subtype antigens representing the world-wide epidemic.METHODS: HIVIS-DNA plasmids which include Env genes of subtypes A, B and C together with Gag subtypes A and B and RTmut/Rev of subtype B were modified as follows: the Envelope sequences were shortened, codon optimized, provided with an FT4 sequence and an immunodominant region mutated. The reverse transcriptase (RT) gene was shortened to contain the most immunogenic N-terminal fragment and fused with an inactivated viral protease vPR gene. HIVISopt-DNA thus contains fewer plasmids but additional PR epitopes compared to the native HIVIS-DNA. DNA components were delivered intradermally to young Balb/c mice once, using a needle-free Biojector® immediately followed by dermal electroporation. Vaccinia-based MVA-CMDR boosts including Env gene E and Gag-RT genes A were delivered intramuscularly by needle, once or twice.RESULTS: Both HIVIS-DNA and HIVISopt-DNA primed humoral and cell mediated responses well. When boosted with heterologous MVA-CMDR (subtypes A and E) virus inhibitory neutralizing antibodies were obtained to HIV-1 subtypes A, B, C and AE. Both plasmid compositions boosted with MVA-CMDR generated HIV-1 specific cellular responses directed against HIV-1 Env, Gag and Pol, as measured by IFNγ ELISpot. It was shown that DNA priming augmented the vector MVA immunological boosting effects, the HIVISopt-DNA with a trend to improved (Env) neutralization, the HIVIS-DNA with a trend to better (Gag) cell mediated immune reponses.CONCLUSIONS: HIVIS-DNA was modified to obtain HIVISopt-DNA that had fewer plasmids, and additional epitopes. Even with one DNA prime followed by two MVA-CMDR boosts, humoral and cell-mediated immune responses were readily induced by priming with either DNA construct composition. Priming by HIV-DNA augmented neutralizing antibody responses revealed by boosting with the vaccinia-based heterologous sequences. Cellular and antibody responses covered selected strains representing HIV-1 subtypes A, B, C and CRF01_AE. We assume this is related to the inclusion of heterologous full genes in the vaccine schedule.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Petkov, SKarolinska Institutet
(författare)
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Ljungberg, KKarolinska Institutet
(författare)
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Hallengärd, DDepartment of Microbiology Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden
(författare)
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Bråve, ADepartment of Microbiology Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden
(författare)
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Isaguliants, MKarolinska Institutet
(författare)
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Falkeborn, TinaLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)tinlu81
(författare)
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Sharma, SumitLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)sumsh85
(författare)
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Liakina, VFaculty of Medicine, Vilnius University 2, 08661 Vilnius, Lithuania
(författare)
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Robb, MU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, 20892 MD, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, 20892 MD, USA
(författare)
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Eller, MU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, 20892 MD, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, 20892 MD, USA
(författare)
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Moss, BLaboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 20892 MD, USA
(författare)
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Biberfeld, GKarolinska Institutet
(författare)
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Sandström, EDepartment of South Hospital, Karolinska Institutet, 11883 Stockholm, Sweden
(författare)
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Nilsson, CKarolinska Institutet
(författare)
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Markland, KClinical Research Center and Vecura, Karolinska University Hospital, 17176 Stockholm, Sweden
(författare)
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Blomberg, PKarolinska Institutet
(författare)
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Wahren, BKarolinska Institutet
(författare)
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Linköpings universitetAvdelningen för mikrobiologi och molekylär medicin
(creator_code:org_t)
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Ingår i:Heliyon: Elsevier3:62405-8440
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Hinkula, Jorma
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Petkov, S
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Ljungberg, K
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Hallengärd, D
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Bråve, A
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Isaguliants, M
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visa fler...
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Falkeborn, Tina
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Sharma, Sumit
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Liakina, V
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Robb, M
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Eller, M
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Moss, B
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Biberfeld, G
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Sandström, E
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Nilsson, C
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Markland, K
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Blomberg, P
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Wahren, B
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