WFRF:(Ahn Ji Young)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03363naa a2200421 4500 | |
| 001 | oai:lup.lub.lu.se:ea7a50e6-006f-4694-a7f7-824cee5b83bb | |
| 003 | SwePub | |
| 008 | 160401s2010 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/16886872 URI |
| 024 | 7 | a https://doi.org/10.1021/pr100300t2 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Ji-Young, Ahn4 aut |
| 245 | 1 0 | a Aptamer microarray mediated capture and mass spectrometry identification of biomarker in serum samples. |
| 264 | c 2010-09-24 | |
| 264 | 1 | b American Chemical Society (ACS),c 2010 |
| 520 | a Sensitive detection of molecular biomarkers in clinical samples is crucially important in disease diagnostics. This paper reports the developement of an aptamer microarray platform combined with sol-gel technology to identify low-abundance targets in complex serum samples. Because of the nanoporous structure of the sol-gel, a high capacity to immobilize the affinity specific aptamers is accomplished which allows binding and detection of target molecules with high sensitivity. The captured protein is digested in situ and the obtained digest was analyzed by ESI-MS without any interference from the affinity probe. TBP (TATA Box Protein) and its specific aptamers were chosen as a model system. A proof of concept with protein concentrations ranging between nanomolar to micromolar is reported, showing a good linearity up to 400 nM when characterized in an aptamer sandwich assay. Moreover, as low as 0.001% of target protein present in total serum proteins could be identified without any pretreatment step using ESI MS/MS mass spectrometry. We believe this novel strategy could become an efficient method for aptamer-based biomarker detection linked directly to mass spectrometry readout. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Medicinteknik0 (SwePub)2062 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Medical Engineering0 (SwePub)2062 hsv//eng |
| 653 | a sol-gel immobilization | |
| 653 | a aptamer microarray | |
| 653 | a sandwich assay | |
| 653 | a biomarkers | |
| 653 | a ESI MS/MS mass spectrometry | |
| 700 | 1 | a Lee, Sang Wooku Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)elma-snl |
| 700 | 1 | a Kang, Hye Suk4 aut |
| 700 | 1 | a Jo, Minjoung4 aut |
| 700 | 1 | a Lee, Dong-Ki4 aut |
| 700 | 1 | a Laurell, Thomasu Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)elma-tla |
| 700 | 1 | a Kim, Soyoun4 aut |
| 710 | 2 | a Avdelningen för Biomedicinsk teknikb Institutionen för biomedicinsk teknik4 org |
| 773 | 0 | t Journal of Proteome Researchd : American Chemical Society (ACS)g 9:11, s. 5568-5573q 9:11<5568-5573x 1535-3893x 1535-3907 |
| 856 | 4 | u http://dx.doi.org/10.1021/pr100300ty FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/1688687 |
| 856 | 4 8 | u https://doi.org/10.1021/pr100300t |
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