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Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle
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- Roepstorff, P. (author)
- Department of Molecular Biology, Odense University, Denmark
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- Højrup, P. (author)
- Department of Molecular Biology, Odense University, Denmark
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- Sundqvist, B.U.R. (author)
- Tandem Accelerator Laboratory, Uppsala University
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- (creator_code:org_t)
- Wiley, 1986
- 1986
- English.
- In: Journal of Mass Spectrometry. - : Wiley. - 1076-5174 .- 1096-9888. ; 13:12, s. 689-691
- Journal article (peer-reviewed)
Abstract
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- During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria, a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS-gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127I-plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS-electrophoresis to be too high.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- ref (subject category)
- art (subject category)
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- Jonsson, Gunnar
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- Andersson, S. O.
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- Johansson, K. E.
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- Karlstad University
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