Response of protein synthesis in human skeletal muscle to insulin: an investigation with L[2H5]phenylalanine

Response of protein synthesis in human skeletal muscle to insulin : an investigation with L[2H5]phenylalanine

McNurlan, Margaret A. (author): Rowett Research Institute, Aberdeen, United Kingdom.

Essén, Pia (author)

Thorell, Anders (author)

Calder, A. Graham (author)

Andersson, S. E. (author)

Ljungqvist, Olle, 1954- (author): Örebro universitet,Institutionen för medicinska vetenskaper

Sandgren, Anna (author)

Grant, Ian (author)

Tjäder, Inga (author)

Ballmer, Peter E. (author)

Wernerman, Jan (author)

Garlick, Peter J. (author)

Rowett Research Institute, Aberdeen, United Kingdom Institutionen för medicinska vetenskaper (creator_code:org_t)

American Physiological Society, 1994
1994
English.
In: American Journal of Physiology. - : American Physiological Society. - 0002-9513 .- 2163-5773. ; 67:Part 1, s. E102-E108

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

The role of insulin in the regulation of muscle protein synthesis in adult humans has been investigated with intravenous infusion of insulin at levels comparable with those observed after normal feeding. Glucose was also infused to maintain euglycemia. Muscle protein synthesis was measured in six healthy subjects before and during insulin and glucose infusion from the incorporation of L-[H-2(5)]phenylalanine into the protein of vastus lateralis sampled by percutaneous biopsy. L-[H-2(5)]phenylalanine was given as a single injection of a flooding amount (45 mg/kg). The relatively low levels of enrichment of phenylalanine in protein (0.005 atom%) were measured by modified gas chromatography-mass spectrometry and verified by comparison with incorporation of L-[2,6-H-3]phenylalanine. Similarity of enrichment in tissue-free and plasma pools (flooding) and linear incorporation over the period of measurement were also verified. The fractional rate of muscle protein synthesis in the group of postabsorptive subjects was 1.65 +/- 0.11% (SE)/day. The rate was unaltered by insulin and glucose infusion, 1.66 +/- 0.16%/day.

By the author/editor: McNurlan, Margar ...; Essén, Pia; Thorell, Anders; Calder, A. Graha ...; Andersson, S. E.; Ljungqvist, Olle ...; show more...; Sandgren, Anna; Grant, Ian; Tjäder, Inga; Ballmer, Peter E ...; Wernerman, Jan; Garlick, Peter J ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.